Liu Dongyou, Lawrence Mark L, Ainsworth A Jerald, Austin Frank W
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS 39762, USA.
Res Microbiol. 2004 Nov;155(9):741-6. doi: 10.1016/j.resmic.2004.05.013.
Listeria seeligeri is a non-pathogenic bacterium coming under the genus Listeria. As this bacterium resembles other Listeria species such as L. monocytogenes and L. ivanovii that are pathogenic to man and animals, it is important that rapid and precise identification techniques be available for L. seeligeri in cases where such determination is desirable. A specific molecular test on the basis of a uniquely present gene region in L. seeligeri will be of particular value under the circumstances. In this report, after comparative screening of genomic DNA from six Listeria species by dot blot hybridization, we isolated one L. seeligeri-specific clone (lse24-315) that contains an insert of 1538 bp. Using primers (lse24-315F and lse24-315R) derived from this clone, we showed that a specific PCR product of 375 bp was generated from genomic DNA of L. seeligeri strains only, but not of other Listeria species or common bacteria. Therefore, the PCR employing primers lse24-315F and lse24-315R provides a rapid, sensitive and specific method for distinguishing L. seeligeri from other Listeria and common bacteria.
海氏李斯特菌是李斯特菌属中的一种非致病性细菌。由于这种细菌与其他对人和动物致病的李斯特菌物种（如单核细胞增生李斯特菌和伊氏李斯特菌）相似，因此在需要进行此类鉴定的情况下，拥有快速且精确的海氏李斯特菌鉴定技术非常重要。在这种情况下，基于海氏李斯特菌中独特存在的基因区域进行的特定分子检测将具有特别的价值。在本报告中，通过斑点印迹杂交对六种李斯特菌物种的基因组DNA进行比较筛选后，我们分离出了一个海氏李斯特菌特异性克隆（lse24 - 315），其插入片段为1538 bp。使用从该克隆衍生的引物（lse24 - 315F和lse24 - 315R），我们发现仅从海氏李斯特菌菌株的基因组DNA中产生了375 bp的特异性PCR产物，而其他李斯特菌物种或常见细菌则没有。因此，使用引物lse24 - 315F和lse24 - 315R的PCR为区分海氏李斯特菌与其他李斯特菌及常见细菌提供了一种快速、灵敏且特异的方法。
