Bobe Julien, Nguyen Thaovi, Jalabert Bernard
Institut National de la Recherche Agronomique, SCRIBE, Campus de Beaulieu, 35042 Rennes Cedex, France.
Biol Reprod. 2004 Jul;71(1):73-82. doi: 10.1095/biolreprod.103.025205. Epub 2004 Feb 25.
A real-time polymerase chain reaction-based gene expression survey was performed using 37 target genes and 22 female rainbow trout sampled during follicular maturational competence (FMC) acquisition or during oocyte maturation. In females sampled before meiosis resumption, FMC was estimated using an in vitro assay. Several growth factors, bone morphogenetic proteins, steroidogenic enzymes, cathepsins, genes known to play a role in the fish preovulatory ovary, as well as previously unstudied genes, were analyzed in this survey. Gene expression profiling was performed using a supervised clustering analysis in order to identify groups of genes exhibiting similar expression profiles in the ovary during FMC acquisition and follicular maturation. From the clustering analysis, three clusters exhibiting a specific expression during FMC acquisition or at the time of oocyte maturation were identified. Cluster 1 was characterized by a progressive increase in gene expression during FMC acquisition, whereas cluster 2 exhibited an increased expression at the time of oocyte maturation. In contrast, cluster 3 was characterized by a decreased mRNA expression at the time of oocyte maturation. Among the 37 target genes used in this survey, 18 were significantly regulated during maturational competence acquisition or at the time of oocyte maturation. Among these 18 genes, 16 belonged to one of the three clusters identified. Although the results allowed a global description of gene expression profiles, they also suggest an important role for several factors, including some previously unstudied bone morphogenetic proteins, in the paracrine control of FMC acquisition and meiosis resumption.
利用37个靶基因，对在卵泡成熟能力（FMC）获得过程中或卵母细胞成熟期间采集的22条雌性虹鳟鱼进行了基于实时聚合酶链反应的基因表达调查。对于在减数分裂恢复前采集的雌性虹鳟鱼，使用体外试验来评估FMC。本调查分析了几种生长因子、骨形态发生蛋白、类固醇生成酶、组织蛋白酶、已知在鱼类排卵前卵巢中起作用的基因以及此前未研究过的基因。为了识别在FMC获得和卵泡成熟期间卵巢中呈现相似表达谱的基因群，使用监督聚类分析进行基因表达谱分析。通过聚类分析，鉴定出在FMC获得期间或卵母细胞成熟时呈现特定表达的三个聚类。聚类1的特征是在FMC获得期间基因表达逐渐增加，而聚类2在卵母细胞成熟时表达增加。相比之下，聚类3的特征是在卵母细胞成熟时mRNA表达下降。在本调查使用的37个靶基因中，有18个在成熟能力获得期间或卵母细胞成熟时受到显著调控。在这18个基因中，有16个属于鉴定出的三个聚类之一。虽然这些结果能够对基因表达谱进行整体描述，但它们也表明包括一些此前未研究过的骨形态发生蛋白在内的几种因子在FMC获得和减数分裂恢复的旁分泌控制中发挥重要作用。
