猫尾草花粉淀粉颗粒在大鼠过敏模型中诱导体液免疫和细胞介导的免疫反应。

Phleum pratense pollen starch granules induce humoral and cell-mediated immune responses in a rat model of allergy.

作者信息

Motta A, Peltre G, Dormans J A M A, Withagen C E T, Lacroix G, Bois F, Steerenberg P A

机构信息

National Institute of Public Health and the Environment, Bilthoven, The Netherlands.

出版信息

Clin Exp Allergy. 2004 Feb;34(2):310-4. doi: 10.1111/j.1365-2222.2004.01872.x.

DOI:10.1111/j.1365-2222.2004.01872.x

PMID:14987313

Abstract

BACKGROUND

Timothy grass (Phleum pratense) pollen allergens are an important cause of allergic symptoms. However, pollen grains are too large to penetrate the deeper airways. Grass pollen is known to release allergen-bearing starch granules (SG) upon contact with water. These granules can create an inhalable allergenic aerosol capable of triggering an early asthmatic response and are implicated in thunderstorm-associated asthma.

OBJECTIVE

We studied the humoral (IgE) and bronchial lymph node cells reactivities to SG from timothy grass pollen in pollen-sensitized rats.

METHODS

Brown-Norway rats were sensitized (day 0) and challenged (day 21) intratracheally with intact pollen and kept immunized by pollen intranasal instillation by 4 weeks intervals during 3 months. Blood and bronchial lymph nodes were collected 7 days after the last intranasal challenge. SG were purified from fresh timothy grass pollen using 5 microm mesh filters. To determine the humoral response (IgE) to SG, we developed an original ELISA inhibition test, based on competition between pollen allergens and purified SG. The cell-mediated response to SG in the bronchial lymph node cells was determined by measuring the uptake of [3H]thymidine in a proliferation assay.

RESULTS

An antibody response to SG was induced, and purified SG were able to inhibit the IgE ELISA absorbance by 45%. Pollen extract and intact pollen gave inhibitions of 55% and 52%, respectively. A cell-mediated response was also found, as pollen extract, intact pollen and SG triggered proliferation of bronchial lymph node cells.

CONCLUSIONS

It was confirmed that timothy grass pollen contains allergen-loaded SG, which are released upon contact with water. These granules were shown to be recognized by pollen-sensitized rats sera and to trigger lymph node cell proliferation in these rats. These data provide new arguments supporting the implication of grass pollen SG in allergic asthma.

摘要

背景

梯牧草（早熟禾）花粉过敏原是过敏症状的一个重要诱因。然而，花粉颗粒太大，无法穿透更深的气道。已知草花粉在与水接触时会释放携带过敏原的淀粉颗粒（SG）。这些颗粒可产生一种可吸入的致敏气溶胶，能够引发早期哮喘反应，并与雷暴相关哮喘有关。

目的

我们研究了花粉致敏大鼠体内体液（IgE）和支气管淋巴结细胞对梯牧草花粉中SG的反应性。

方法

用完整花粉对棕色挪威大鼠进行气管内致敏（第0天）和激发（第21天），并在3个月内每隔4周通过鼻内滴注花粉使其保持免疫状态。在最后一次鼻内激发后7天收集血液和支气管淋巴结。使用5微米筛网过滤器从新鲜梯牧草花粉中纯化SG。为了确定对SG的体液反应（IgE），我们基于花粉过敏原与纯化的SG之间的竞争开发了一种原始的ELISA抑制试验。通过在增殖试验中测量[3H]胸腺嘧啶核苷的摄取来确定支气管淋巴结细胞对SG的细胞介导反应。