Andreu Núria, Soto Carlos Y, Roca Ignasi, Martín Carlos, Gibert Isidre
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain.
FEMS Microbiol Lett. 2004 Feb 16;231(2):283-9. doi: 10.1016/S0378-1097(04)00008-4.
Neutral red staining is a cytochemical reaction that has been found to be related to Mycobacterium tuberculosis virulence and, therefore, the component involved in it is thought to be a virulence factor. To study the molecular basis of this reaction we constructed an M. tuberculosis cosmid library in Mycobacterium smegmatis and selected recombinant neutral red positive clones. Heterologous complementation identified Rv0577 as the gene responsible for this trait and we have also shown that it is expressed as a single polycistronic unit together with Rv0576 which could also be involved in the neutral red staining.
中性红染色是一种细胞化学反应，已发现其与结核分枝杆菌的毒力有关，因此，参与其中的成分被认为是一种毒力因子。为了研究这种反应的分子基础，我们构建了一个耻垢分枝杆菌中的结核分枝杆菌黏粒文库，并筛选出重组中性红阳性克隆。异源互补鉴定出Rv0577是负责该性状的基因，并且我们还表明它与Rv0576一起作为单个多顺反子单元表达，Rv0576也可能参与中性红染色。
