Piskur Jure, Sandrini Michael P B, Knecht Wolfgang, Munch-Petersen Birgitte
BioCentrum-DTU, Eukaryote Molecular Biology Group, Building 301, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
FEBS Lett. 2004 Feb 27;560(1-3):3-6. doi: 10.1016/S0014-5793(04)00081-X.
Deoxyribonucleoside kinases, which catalyse the phosphorylation of deoxyribonucleosides, are present in several copies in most multicellular organisms and therefore represent an excellent model to study gene duplication and specialisation of the duplicated copies through partitioning of substrate specificity. Recent studies suggest that in the animal lineage one of the progenitor kinases, the so-called dCK/dGK/TK2-like gene, was duplicated prior to separation of the insect and mammalian lineages. Thereafter, insects lost all but one kinase, dNK (EC 2.7.1.145), which subsequently, through remodelling of a limited number of amino acid residues, gained a broad substrate specificity.
脱氧核糖核苷激酶催化脱氧核糖核苷的磷酸化反应，在大多数多细胞生物中以多个拷贝形式存在，因此是研究基因复制以及通过底物特异性划分实现复制拷贝特化的绝佳模型。最近的研究表明，在动物谱系中，一种祖先进化激酶，即所谓的dCK/dGK/TK2样基因，在昆虫和哺乳动物谱系分离之前就发生了复制。此后，昆虫除了一种激酶dNK（EC 2.7.1.145）外，其余激酶全部丢失，随后dNK通过有限数量氨基酸残基的重塑，获得了广泛的底物特异性。
