Ogawa Y, Orts W J, Glenn G M, Wood D F
USDA, ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
Biotech Histochem. 2003 Oct;78(5):237-42. doi: 10.1080/10520290310001630467.
We developed a new and simple method to collect sections of a whole brown rice kernel for investigation of histological properties. A single kernel of rice was dehydrated through a graded ethanol series, transferred to xylene, and embedded in paraffin. During sectioning of the blocks using a rotary microtome, we used a special adhesive tape to collect and place the sections on slides so they remained flat. The use of the adhesive tape technique combined with autofluorescence characteristics allowed us to visualize cell walls throughout an entire longitudinal or transverse section of a whole rice kernel. We obtained scanning electron microscopy images of the sections to determine section quality.
我们开发了一种新的简单方法来收集完整糙米籽粒的切片，用于组织学特性研究。将一粒水稻籽粒通过梯度乙醇系列脱水，转移至二甲苯中，然后嵌入石蜡。在使用旋转切片机对蜡块进行切片时，我们使用一种特殊的胶带收集切片并将其放置在载玻片上，使其保持平整。胶带技术与自发荧光特性相结合，使我们能够在整个糙米籽粒的纵向或横向切片中观察到细胞壁。我们获取了切片的扫描电子显微镜图像以确定切片质量。
