Wang Feng, Wang Chen, Wang Tie, Pang Bao-sen, Wu Yan-bing, Yang Yuan-hua, Li Chong, Zhang Hong-yu, Weng Xin-zhi
Beijing Institute of Respiratory Medicine-Beijing Chaoyang Hospital, Capital University of Medical Sciences, Beijing 100020, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2004 Feb;27(2):93-6.
To compare the thrombolytic effects of the two dosing regimes with urokinase (UK) in a canine model of pulmonary thromboembolism induced by radioactive blood clots.
Seventeen dogs were randomly assigned into three groups: the control group, the UK(2h) group (UK infused over 2 hours) and the UK(12h) group (UK given over 12 hours). The thrombolytic differences was investigated among the three groups. Thrombolysis was assessed by continuously counting over both lung fields with single photon emission computed tomography (SPECT) and calculated by regions of interest (ROI) technology and by counting radioactivity in the lung in vitro. The extent of thrombolysis was calculated as the difference between the radioactivity originally incorporated in the clot (decay-corrected) and the radioactivity in the lung in vitro.
In three groups, the lysis rates measured by ROI technology were (6.2 +/- 4.0)%, (39.5 +/- 13.9)%, and (16.9 +/- 8.9)% respectively, and (6.0 +/- 2.7)%, (42.8 +/- 12.4)%, and (17.7 +/- 9.3)% by the method in vivo. The thrombolytic ratio of the UK(2h) group was significantly higher than that of the other two groups (P < 0.01), and there was no marked difference between the control group and the UK(12h) group. There was a thrombolytic peak in the UK(2h) group at the first four hours after infusion of agent.
For the fresh thrombi, the UK(2h) regime is superior to the UK(12h) due to its higher thrombolytic ratio and prompt thrombolytic property. The model and methods are highly reliable.
在放射性血凝块诱导的犬肺血栓栓塞模型中比较两种尿激酶（UK）给药方案的溶栓效果。
将17只犬随机分为三组：对照组、UK（2小时）组（尿激酶输注2小时）和UK（12小时）组（尿激酶给药12小时）。研究三组之间的溶栓差异。通过单光子发射计算机断层扫描（SPECT）对双肺野进行连续计数，并采用感兴趣区（ROI）技术计算，以及通过体外测定肺内放射性来评估溶栓情况。溶栓程度计算为最初凝块中所含放射性（经衰变校正）与体外肺内放射性之间的差值。
三组中，采用ROI技术测量的溶解率分别为（6.2±4.0）%、（39.5±13.9）%和（16.9±8.9）%，体内方法测量的溶解率分别为（6.0±2.7）%、（42.8±12.4）%和（17.7±9.3）%。UK（2小时）组的溶栓率显著高于其他两组（P<0.01），对照组与UK（12小时）组之间无明显差异。UK（2小时）组在给药后前4小时出现溶栓高峰。
对于新鲜血栓，UK（2小时）给药方案因其较高的溶栓率和快速的溶栓特性优于UK（12小时）给药方案。该模型和方法具有高度可靠性。
