Nakamura Koichi, Inaba Muneo, Sugiura Kikuya, Yoshimura Tomoo, Kwon A-Hon, Kamiyama Yasuo, Ikehara Susumu
First Department of Pathology, Kansai Medical University, Osaka, Japan.
Stem Cells. 2004;22(2):125-34. doi: 10.1634/stemcells.22-2-125.
We examined the effect of intra-bone marrow (IBM)-bone marrow transplantation (BMT) in conjunction with donor lymphocyte infusion (DLI) on the engraftment of allogeneic bone marrow cells (BMCs) in mice. Recipients that had received 6 Gy of radiation completely rejected donor BMCs, even when IBM-BMT was carried out. However, when BMCs were IBM injected and donor peripheral blood mononuclear cells (PBMNCs) were simultaneously injected intravenously (DLI), donor cell engraftment was observed 7 days after BMT and complete donor chimerism continued thereafter. It is of interest that the cells of recipient origin did not recover, and that the hematolymphoid cells, including progenitor cells (Lin-/c-kit+ cells) in the recipients, were fully reconstituted with cells of donor origin. The cells in the PBMNCs responsible for the donor BMC engraftment were CD8+. Recipients that had received 6 Gy of radiation, IBM-BMT, and DLI showed only a slight loss of body weight, due to radiation side effects, and had no macroscopic or microscopic symptoms of graft-versus-host disease. These findings suggest that IBM-BMT in conjunction with DLI will be a valuable strategy for allogeneic BMT in humans.
我们研究了骨髓内（IBM）骨髓移植（BMT）联合供体淋巴细胞输注（DLI）对小鼠同种异体骨髓细胞（BMCs）植入的影响。接受6 Gy辐射的受体完全排斥供体BMCs，即使进行了IBM-BMT也是如此。然而，当将BMCs进行骨髓内注射并同时静脉注射供体外周血单个核细胞（PBMNCs）（DLI）时，在BMT后7天观察到供体细胞植入，此后持续出现完全的供体嵌合现象。有趣的是，受体来源的细胞没有恢复，并且受体中的血液淋巴细胞，包括祖细胞（Lin-/c-kit+细胞），完全由供体来源的细胞重建。PBMNCs中负责供体BMC植入的细胞是CD8+。接受6 Gy辐射、IBM-BMT和DLI的受体仅因辐射副作用而出现轻微体重减轻，且没有移植物抗宿主病的宏观或微观症状。这些发现表明，IBM-BMT联合DLI将是人类同种异体BMT的一种有价值的策略。
