Eggan Kevin, Baldwin Kristin, Tackett Michael, Osborne Joseph, Gogos Joseph, Chess Andrew, Axel Richard, Jaenisch Rudolf
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
Nature. 2004 Mar 4;428(6978):44-9. doi: 10.1038/nature02375. Epub 2004 Feb 15.
Cloning by nuclear transplantation has been successfully carried out in various mammals, including mice. Until now mice have not been cloned from post-mitotic cells such as neurons. Here, we have generated fertile mouse clones derived by transferring the nuclei of post-mitotic, olfactory sensory neurons into oocytes. These results indicate that the genome of a post-mitotic, terminally differentiated neuron can re-enter the cell cycle and be reprogrammed to a state of totipotency after nuclear transfer. Moreover, the pattern of odorant receptor gene expression and the organization of odorant receptor genes in cloned mice was indistinguishable from wild-type animals, indicating that irreversible changes to the DNA of olfactory neurons do not accompany receptor gene choice.
通过核移植进行克隆已在包括小鼠在内的各种哺乳动物中成功实现。到目前为止，还没有从小鼠的有丝分裂后细胞（如神经元）克隆出小鼠。在此，我们通过将有丝分裂后嗅觉感觉神经元的细胞核转移到卵母细胞中，成功培育出了可育的小鼠克隆体。这些结果表明，有丝分裂后、终末分化的神经元基因组在核移植后可以重新进入细胞周期并被重编程为全能状态。此外，克隆小鼠中气味受体基因的表达模式和气味受体基因的组织与野生型动物没有区别，这表明嗅觉神经元DNA的不可逆变化与受体基因选择无关。
