包含AFLP、SSR、ESTP、5S rDNA和形态学标记的欧洲云杉全饱和连锁图谱。
A full saturated linkage map of Picea abies including AFLP, SSR, ESTP, 5S rDNA and morphological markers.
作者信息
Acheré V, Faivre-Rampant P, Jeandroz S, Besnard G, Markussen T, Aragones A, Fladung M, Ritter E, Favre J-M
机构信息
UMR INRA/UHP 1136, Tree-Microbe Interactions, Faculté des Sciences, Université Nancy I, BP 239, 54506 Vandoeuvre-lès-Nancy, France.
出版信息
Theor Appl Genet. 2004 May;108(8):1602-13. doi: 10.1007/s00122-004-1587-y. Epub 2004 Feb 27.
Based on an F(1) progeny of 73 individuals, two parental maps were constructed according to the double pseudo-test cross strategy. The paternal map contained 16 linkage groups for a total genetic length of 1,792 cM. The maternal map covered 1,920 cM, and consisted of 12 linkage groups. These parental maps were then integrated using 66 intercross markers. The resulting consensus map covered 2,035 cM and included 755 markers (661 AFLPs, 74 SSRs, 18 ESTPs, the 5S rDNA and the early cone formation trait) on 12 linkage groups, reflecting the haploid number of chromosomes of Picea abies. The average spacing between two adjacent markers was 2.6 cM. The presence of 39 of the SSR and/or ESTP markers from this consensus map on other published maps of different Picea and Pinus species allowed us to establish partial linkage group homologies across three P. abies maps (up to five common markers per linkage group). This first saturated linkage map of P. abies could be therefore used as a support for developing comparative genome mapping in conifers.
基于一个由73个个体组成的F(1)子代，根据双假测交策略构建了两个亲本图谱。父本图谱包含16个连锁群，总遗传长度为1792厘摩。母本图谱覆盖1920厘摩，由12个连锁群组成。然后使用66个杂交标记对这些亲本图谱进行整合。得到的共识图谱覆盖2035厘摩，包含12个连锁群上的755个标记（661个扩增片段长度多态性标记、74个简单序列重复标记、18个表达序列标签多态性标记、5S核糖体DNA和早期球果形成性状），反映了欧洲云杉染色体的单倍体数目。两个相邻标记之间的平均间距为2.6厘摩。该共识图谱上39个简单序列重复标记和/或表达序列标签多态性标记存在于其他已发表的不同云杉和松树物种图谱上，这使我们能够在三张欧洲云杉图谱之间建立部分连锁群同源性（每个连锁群最多有五个共同标记）。因此，这第一张欧洲云杉饱和连锁图谱可作为开展针叶树比较基因组图谱研究的支撑。
Zotero 插件