Hao Zhuan-Fang, Li Xin-Hai, Zhang Shi-Huang
Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Yi Chuan Xue Bao. 2005 Sep;32(9):960-8.
Simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs) have become the most important markers for molecular mapping. Primarily based on restriction fragment length polymorphism (RFLP) markers, extensive linkage maps of maize had been developed. To construct a near-saturated genetic linkage map, an expanded maize genetic linkage map was constructed using a population of 234 F2 individuals derived from a cross of X178 and B73 base on an essential SSR framework map of maize. The level of polymorphisms and genetic properties of SSR and AFLP markers were characterized. A total of 249 markers consisting of 130 SSRs and 119 AFLPs have been landed on 10 chromosomes of maize. The 249-locus map spanned 1 659.3 centi-morgans (cM) and had a mean density of 6.66 cM. This mapping population and related information should connect further research involving analysis of quantitative trait loci, comparative genomics, and heterosis. Moreover, in many studies, AFLPs were analyzed on the basis of the presence or absence of a band on the electrophoresis gels. A new method based on double polymorphic bands of co-dominant scoring of AFLPs was explored according to the similarity of loci amplified from AFLP enzyme combination.
简单序列重复（SSRs）和扩增片段长度多态性（AFLPs）已成为分子图谱构建中最重要的标记。基于限制性片段长度多态性（RFLP）标记，已构建了玉米的广泛连锁图谱。为构建一个近乎饱和的遗传连锁图谱，基于玉米的一个基本SSR框架图谱，利用由X178和B73杂交产生的234个F2个体群体构建了一个扩展的玉米遗传连锁图谱。对SSR和AFLP标记的多态性水平和遗传特性进行了表征。共有249个标记（包括130个SSR和119个AFLP）定位到玉米的10条染色体上。这个包含249个位点的图谱跨度为1659.3厘摩（cM），平均密度为6.66 cM。这个作图群体及相关信息将为进一步开展涉及数量性状位点分析、比较基因组学和杂种优势等方面的研究提供联系。此外，在许多研究中，AFLPs是基于电泳凝胶上条带的有无进行分析的。根据从AFLP酶切组合扩增出的位点的相似性，探索了一种基于AFLPs共显性评分的双多态性条带的新方法。
