Kozhura V L, Tlatova T A, Kondakova N V, Sakharova V V, Ripa N V
Anesteziol Reanimatol. 2003 Nov-Dec(6):21-3.
The citogenetic lesions were evaluated in the marrow erythroblasts of 45 anesthetized white nonlinear male rats, weight--200-300 g who were subjected to an acute blood loss with a 1-hour arterial hypotension (ABR = 40 mm Hg); the micronucleus tests was made use of. Two stages of the increase of polychromatophilic erythrocytes with micronuclei in the marrow of the animals, who underwent a massive blood loss, were registered: stage 1--an incomplete marrow ischemia with a subsequent arterial hypotension and with a reliably confirmed formation of cytogenetic lesions in the marrow polychromatophilic erythrocytes; stage 2--the reperfusion period contributed to a 1.7-fold increase of polychromatophilic erythrocytes with micronuclei versus the previous stage. Mexidole, when used at 50 mg/kg prior to blood reinfusion, decreased the quantity of polychromatophilic erythrocytes with micronuclei to the basic level, which is indicative of reversibility and instability of cytogenetics impairments in the marrow cells of animals observed in the early post-resuscitation period.
对45只体重200 - 300克、处于麻醉状态的白色非线性雄性大鼠的骨髓成红细胞中的细胞遗传学损伤进行了评估，这些大鼠经历了急性失血并伴有1小时的动脉低血压（ABR = 40毫米汞柱）；采用了微核试验。记录了经历大量失血的动物骨髓中含微核的多染性红细胞增加的两个阶段：第1阶段——不完全性骨髓缺血，随后出现动脉低血压，且骨髓多染性红细胞中细胞遗传学损伤的形成得到可靠证实；第2阶段——再灌注期导致含微核的多染性红细胞数量比前一阶段增加了1.7倍。在输血前以50毫克/千克的剂量使用美西多宁，可使含微核的多染性红细胞数量降至基础水平，这表明在复苏后早期观察到的动物骨髓细胞中细胞遗传学损伤具有可逆性和不稳定性。
