四川产六种药用姜黄属植物的分子鉴定：来自叶绿体trnK基因序列的证据

Molecular identification of six medicinal Curcuma plants produced in Sichuan: evidence from plastid trnK gene sequences.

作者信息

Cao Hui, Komatsu Katsuko

机构信息

National Engineering Research Center for Modernization of Traditional Chinese Medicine, Zhuhai 519020, China.

出版信息

Yao Xue Xue Bao. 2003 Nov;38(11):871-5.

PMID:14992005

Abstract

AIM

To establish a rapid and simple molecular identification method for six medicinals: Curcuma: C. longa, C. phaeocaulis, C. sichuanensis, C. chuanyujin, C. chuanhuangjiang, and C. chuanezhu in Sichuan Province.

METHODS

A molecular approach (trnK nucleotide sequencing) was used in this study.

RESULTS

The sequenced entire chloroplast trnK gene region spanned 2,699-2,705 bp. The matK gene (an intron embodied in trnK gene) sequence and the intron spacer region of the trnK gene have great diversity within these six medicinal Curcuma species. There were six single bases substitutions between trnK coding region and matK region, the 9-bp deletion and 4-bp or 14-bp insertion repeat at some sites of matK region in each taxon.

CONCLUSION

These relatively variable sequences were potentially informative in the identification for these six Curcuma species at the DNA level.

摘要

目的

建立一种快速简便的分子鉴定方法，用于鉴定六种药用植物：姜黄属的长柄姜黄、蓬莪术、川郁金、川黄姜、川鄂姜花和四川莪术。

方法

本研究采用分子方法（trnK核苷酸测序）。

结果

测序得到的叶绿体trnK基因全长区域为2699 - 2705 bp。matK基因（trnK基因中的一个内含子）序列以及trnK基因的内含子间隔区在这六种姜黄属药用植物中具有很大的多样性。trnK编码区与matK区之间存在六个单碱基替换，每个分类单元的matK区某些位点存在9 bp的缺失以及4 bp或14 bp的插入重复。