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鸡(家鸡)离体灌注肝脏中细胞内蛋白质降解的调节

Regulation of intracellular protein degradation in the isolated perfused liver of the chicken (Gallus domesticus).

作者信息

Sakhri M, Jeacock M K, Shepherd D A

机构信息

Department of Biochemistry and Physiology, University of Reading, Whiteknights, UK.

出版信息

Comp Biochem Physiol B. 1992 Jan-Feb;101(1-2):17-21. doi: 10.1016/0305-0491(92)90152-h.

DOI:10.1016/0305-0491(92)90152-h
PMID:1499264
Abstract
  1. The effects of insulin, glucagon and a supply of exogenous amino acids on protein degradation have been studied in isolated perfused livers from growing chickens by measuring the rate of net valine release in the presence of cycloheximide. 2. Insulin inhibited protein degradation as did a supply of exogenous amino acids. 3. Addition of glucagon increased uric acid release from the livers but had no significant effect on protein degradation. 4. When the effects of the hormones and amino acid mixture are compared with published data for the rat it is evident that the action of glucagon differs in the two species.
摘要
  1. 通过在放线菌酮存在的情况下测量缬氨酸净释放速率,研究了胰岛素、胰高血糖素和外源性氨基酸供应对生长鸡离体灌注肝脏中蛋白质降解的影响。2. 胰岛素抑制蛋白质降解,外源性氨基酸供应也有同样作用。3. 添加胰高血糖素可增加肝脏尿酸释放,但对蛋白质降解无显著影响。4. 当将激素和氨基酸混合物的作用与已发表的大鼠数据进行比较时,很明显胰高血糖素在这两个物种中的作用有所不同。

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1
Regulation of intracellular protein degradation in the isolated perfused liver of the chicken (Gallus domesticus).鸡(家鸡)离体灌注肝脏中细胞内蛋白质降解的调节
Comp Biochem Physiol B. 1992 Jan-Feb;101(1-2):17-21. doi: 10.1016/0305-0491(92)90152-h.
2
Effects of glucagon on general protein degradation and synthesis in perfused rat liver.胰高血糖素对灌注大鼠肝脏中总蛋白质降解和合成的影响。
J Biol Chem. 1974 Sep 10;249(17):5458-63.
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Effects of cycloheximide on protein degradation and gluconeogenesis in the perfused rat liver.放线菌酮对灌注大鼠肝脏中蛋白质降解和糖异生的影响。
Biochim Biophys Acta. 1976 Jan 14;421(1):70-9. doi: 10.1016/0304-4165(76)90170-7.
4
Application of liver perfusion as an in vitro model in studies of intracellular protein degradation.肝脏灌注作为体外模型在细胞内蛋白质降解研究中的应用。
Ciba Found Symp. 1979(75):281-305. doi: 10.1002/9780470720585.ch17.
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Influence of amino acid supply on ribosomes and protein synthesis of perfused rat liver.氨基酸供应对灌注大鼠肝脏核糖体及蛋白质合成的影响。
Biochem J. 1969 Mar;111(5):703-12. doi: 10.1042/bj1110703.
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Mechanism and control of protein and RNA degradation in the rat hepatocyte: two modes of autophagic sequestration.大鼠肝细胞中蛋白质和RNA降解的机制与调控:自噬隔离的两种模式。
Revis Biol Celular. 1989;20:79-96.
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Effects of insulin, amino acids and fasting on myofibrillar protein degradation in perfused hindquarters of rats.胰岛素、氨基酸和禁食对大鼠灌注后肢肌原纤维蛋白降解的影响。
J Nutr Sci Vitaminol (Tokyo). 1985 Aug;31(4):431-40. doi: 10.3177/jnsv.31.431.
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Assessment of protein turnover in perfused rat liver. Evidence for amino acid compartmentation from differential labeling of free and tRNA-gound valine.灌注大鼠肝脏中蛋白质周转的评估。游离缬氨酸和tRNA结合缬氨酸差异标记表明氨基酸存在区室化的证据。
J Biol Chem. 1976 Mar 10;251(5):1375-84.
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Amino acid and hormonal control of macromolecular turnover in perfused rat liver. Evidence for selective autophagy.灌注大鼠肝脏中大分子周转的氨基酸和激素控制。选择性自噬的证据。
J Biol Chem. 1987 Oct 25;262(30):14514-9.
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Effects of insulin on amino acid release and urea formation in perfused rat liver.胰岛素对灌注大鼠肝脏中氨基酸释放及尿素形成的影响。
Am J Physiol. 1967 Jan;212(1):173-8. doi: 10.1152/ajplegacy.1967.212.1.173.