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Cathepsin H was purified about 5400-fold from hepatopancreas of carp (Cyprinus carpio) by the method involving ammonium sulfate fractionation, and chromatography on S-Sepharose, DEAE-Sephacel, Ultrogel AcA54, Concanavalin A-Sepharose 4B and GPC on Protein-Pak 125. 2. The purified cathepsin H gave a single protein band on analytical-PAGE, but migrated as two bands of 27,000 and 23,000 mol. wt on SDS-PAGE. 3. Cathepsin H had a pH and temperature optimum of 6.5 and 45 degrees C using Arg-MCA as a substrate, respectively, and was activated by sulfhydryl compounds and inhibited by cysteine protease inhibitors and metal compounds having high reactivities at cysteine residue. 4. The carp hepatopancreas cathepsin H immunoreacted with the monospecific antibody against rat liver cathepsin H, and did not react with the antibodies against carp hepatopancreas cathepsins B and L by the method of immunoelectrophoretic blotting.

通过硫酸铵分级分离以及在S-Sepharose、DEAE-Sephacel、Ultrogel AcA54、伴刀豆球蛋白A-Sepharose 4B上的色谱分离和在Protein-Pak 125上的凝胶渗透色谱法，从鲤鱼（Cyprinus carpio）的肝胰腺中纯化出组织蛋白酶H，纯化倍数约为5400倍。2. 纯化后的组织蛋白酶H在分析型聚丙烯酰胺凝胶电泳上呈现单一蛋白条带，但在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上迁移为两条分子量分别为27,000和23,000的条带。3. 以精氨酸-甲基香豆素酰胺为底物时，组织蛋白酶H的最适pH值和温度分别为6.5和45℃，它可被巯基化合物激活，并被半胱氨酸蛋白酶抑制剂以及在半胱氨酸残基处具有高反应活性的金属化合物抑制。4. 通过免疫电泳印迹法，鲤鱼肝胰腺组织蛋白酶H与抗大鼠肝脏组织蛋白酶H的单特异性抗体发生免疫反应，而不与抗鲤鱼肝胰腺组织蛋白酶B和L的抗体发生反应。

Cathepsin H was purified about 5400-fold from hepatopancreas of carp (Cyprinus carpio) by the method involving ammonium sulfate fractionation, and chromatography on S-Sepharose, DEAE-Sephacel, Ultrogel AcA54, Concanavalin A-Sepharose 4B and GPC on Protein-Pak 125. 2. The purified cathepsin H gave a single protein band on analytical-PAGE, but migrated as two bands of 27,000 and 23,000 mol. wt on SDS-PAGE. 3. Cathepsin H had a pH and temperature optimum of 6.5 and 45 degrees C using Arg-MCA as a substrate, respectively, and was activated by sulfhydryl compounds and inhibited by cysteine protease inhibitors and metal compounds having high reactivities at cysteine residue. 4. The carp hepatopancreas cathepsin H immunoreacted with the monospecific antibody against rat liver cathepsin H, and did not react with the antibodies against carp hepatopancreas cathepsins B and L by the method of immunoelectrophoretic blotting.

通过硫酸铵分级分离以及在S-Sepharose、DEAE-Sephacel、Ultrogel AcA54、伴刀豆球蛋白A-Sepharose 4B上的色谱分离和在Protein-Pak 125上的凝胶渗透色谱法，从鲤鱼（Cyprinus carpio）的肝胰腺中纯化出组织蛋白酶H，纯化倍数约为5400倍。2. 纯化后的组织蛋白酶H在分析型聚丙烯酰胺凝胶电泳上呈现单一蛋白条带，但在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上迁移为两条分子量分别为27,000和23,000的条带。3. 以精氨酸-甲基香豆素酰胺为底物时，组织蛋白酶H的最适pH值和温度分别为6.5和45℃，它可被巯基化合物激活，并被半胱氨酸蛋白酶抑制剂以及在半胱氨酸残基处具有高反应活性的金属化合物抑制。4. 通过免疫电泳印迹法，鲤鱼肝胰腺组织蛋白酶H与抗大鼠肝脏组织蛋白酶H的单特异性抗体发生免疫反应，而不与抗鲤鱼肝胰腺组织蛋白酶B和L的抗体发生反应。

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