Iwasaki Wakana, Miyatake Hideyuki, Ebihara Akio, Miki Kunio
RIKEN Harima Institute/SPring-8, Koto 1-1-1, Mikazuki-cho, Sayo-gun, Hyogo 679-5148, Japan.
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):515-7. doi: 10.1107/S0907444904000721. Epub 2004 Feb 25.
Glucose-inhibited division protein A (GidA) acts in tRNA modification. It has been suggested that GidA is involved in the biosynthesis of the hypermodified nucleotide 5-methylaminomethyl-2-thiouridine in the wobble position of bacterial tRNAs, which stabilizes codon-anticodon interactions. Thermus thermophilus HB8 has a putative small gidA gene in addition to the normal gidA gene. The crystallization and preliminary X-ray crystallographic studies of the product of this small gidA gene (GidA(small)) are reported here. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 78.51, c = 66.10 A and one monomer per asymmetric unit. The crystals were found to diffract X-rays to beyond 1.65 A resolution.
葡萄糖抑制分裂蛋白A(GidA)参与tRNA修饰。有人提出,GidA参与细菌tRNA摆动位置上超修饰核苷酸5-甲基氨基甲基-2-硫代尿苷的生物合成,该修饰可稳定密码子-反密码子相互作用。嗜热栖热菌HB8除了正常的gidA基因外,还有一个假定的小gidA基因。本文报道了这个小gidA基因产物(GidA(small))的结晶及初步X射线晶体学研究。晶体属于空间群P3(1)21或P3(2)21,晶胞参数a = b = 78.51,c = 66.10 Å,每个不对称单元中有一个单体。发现这些晶体对X射线的衍射分辨率超过1.65 Å。