Ishii N, Saijo S, Sato T, Tanaka N, Harata K
Biophysical Chemistry Laboratory, National Institute of Bioscience and Human Technology, 1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
J Struct Biol. 2001 Apr;134(1):88-92. doi: 10.1006/jsbi.2001.4358.
Crystals have been grown of the V(1)-ATPase sector of the V-type ATP synthase complex (V(0)V(1)) from the thermophilic eubacterium Thermus thermophilus HB8. These crystals are grown by the vapor diffusion method in the presence of 5 mM Mg-ADP, from solutions containing 100 mM sodium acetate and 2 M sodium formate, pH 5.5. The crystals diffracted X rays beyond 3.4 A in resolution on a synchrotron radiation source. The crystals belong to the trigonal space group P3, with unit cell dimensions of a = b = 89.0 A, c = 179.2 A, and gamma = 120 degrees. The unit cell presumably contains one molecule of V(1)-ATPase and the V(m) value is calculated as 3.0 A(3)/Da.
已从嗜热真细菌嗜热栖热菌HB8中培养出V型ATP合酶复合物（V(0)V(1)）的V(1)-ATP酶部分的晶体。这些晶体通过气相扩散法在5 mM Mg-ADP存在下，从含有100 mM醋酸钠和2 M甲酸钠、pH 5.5的溶液中生长而成。这些晶体在同步辐射源上的X射线衍射分辨率超过3.4 Å。晶体属于三方晶系空间群P3，晶胞参数为a = b = 89.0 Å，c = 179.2 Å，γ = 120°。晶胞中可能包含一个V(1)-ATP酶分子，V(m)值计算为3.0 Å(3)/Da。
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