Razeto Adelia, Pfitzner Edith, Becker Stefan
Department for NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany.
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):550-2. doi: 10.1107/S0907444903029378. Epub 2004 Feb 25.
Signal transducer and activator of transcription 6 (STAT6) regulates transcriptional activation in response to interleukin-4 (IL-4) by direct interaction with coactivators. Among them, NCoA-1, a member of the p160/steroid receptor coactivator (SRC) family, has been found to bind to STAT6 with the region B of its putative Per-Arnt-Sim (PAS) domain. STAT6 interacts specifically with NCoA-1 via an LXXLL motif in its transactivation domain. Crystals of the NCoA-1(257-385) domain in complex with the STAT6(794-814) LXXLL motif were obtained in two hexagonal space groups. The crystals in space group P6(1), with unit-cell parameters a = 61.7, b = 61.7, c = 146.5 A, alpha = beta = 90, gamma = 120 degrees, diffract to 2.8 A at a home source. Crystals belonging to space group P6(2), with unit-cell parameters a = 62.0, b = 62.0, c = 73.6 A, alpha = beta = 90, gamma = 120 degrees, diffract to 1.8 A at a synchrotron source.
信号转导子和转录激活子6（STAT6）通过与共激活因子直接相互作用来调节对白介素-4（IL-4）的转录激活。其中，p160/类固醇受体共激活因子（SRC）家族成员NCoA-1已被发现通过其假定的Per-Arnt-Sim（PAS）结构域的B区域与STAT6结合。STAT6通过其反式激活结构域中的LXXLL基序与NCoA-1特异性相互作用。NCoA-1（257-385）结构域与STAT6（794-814）LXXLL基序复合物的晶体在两个六方空间群中获得。空间群为P6(1)的晶体，其晶胞参数a = 61.7，b = 61.7，c = 146.5 Å，α = β = 90，γ = 120°，在家庭光源下衍射至2.8 Å。属于空间群P6(2)的晶体，其晶胞参数a = 62.0，b = 62.0，c = 73.6 Å，α = β = 90，γ = 120°，在同步辐射光源下衍射至1.8 Å。
