Beis Konstantinos, Nesper Jutta, Whitfield Chris, Naismith James H
Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland.
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):558-60. doi: 10.1107/S0907444903029494. Epub 2004 Feb 25.
A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 94.6, b = 215.5, c = 218.5 A. A data set to 3.0 A with 99.8% completeness and an R(merge) of 10.5% has been collected from a single crystal.
已纯化并结晶出一种来自大肠杆菌O9a:K30血清型的新型整合膜脂蛋白Wza。Wza是大肠杆菌K30 1型荚膜多糖在表面表达所必需的；在其他产生细胞外多糖的细菌中也发现了密切相关的同源物。Wza晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数a = 94.6，b = 215.5，c = 218.5 Å。已从一块单晶收集到分辨率为3.0 Å的数据集，完整性为99.8%，合并R值为10.5%。
