克拉克芽孢杆菌γ型环糊精糖基转移酶的结晶及X射线初步研究
Crystallization and preliminary X-ray study of gamma-type cyclodextrin glycosyltransferase from Bacillus clarkii.
作者信息
Akita Masatake, Hatada Yuji, Hidaka Yuko, Ohta Yukari, Takada Masayasu, Nakagawa Yoshinori, Ogawa Koichi, Nakakuki Teruo, Ito Susumu, Horikoshi Koki
机构信息
Japan Marine Science and Technology Center, 2-15 Natsushima, Yokosuka 237-0061, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):586-7. doi: 10.1107/S0907444904001076. Epub 2004 Feb 25.
A gamma-cyclodextrin glycosyltransferase (EC 2.4.1.19) from Bacillus clarkii was crystallized using the hanging-drop vapour-diffusion method at 293 K. X-ray diffraction data were collected to 2.2 A. The crystal belongs to space group R3, with unit-cell parameters a = b = 211.6, c = 52.7 A. The asymmetric unit contains one protein molecule, with a corresponding V(M) of 3.03 A(3) Da(-1) and a solvent content of 59.4%. Molecular replacement was successfully carried out using a homology model based on the three-dimensional structure of the CGTase from Thermonanaerobacterium thermosulfurigenes EM1 as a search model.
利用悬滴气相扩散法在293 K下对来自克拉克芽孢杆菌的γ-环糊精糖基转移酶（EC 2.4.1.19）进行了结晶。收集到了分辨率为2.2 Å的X射线衍射数据。晶体属于R3空间群，晶胞参数a = b = 211.6，c = 52.7 Å。不对称单元包含一个蛋白质分子，其对应的V(M)为3.03 ų Da⁻¹，溶剂含量为59.4%。以嗜热栖热硫化叶菌EM1的CGTase三维结构为搜索模型，基于同源性模型成功进行了分子置换。
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