来自吉氏纤维弧菌的纤维二糖磷酸化酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of cellobiose phosphorylase from Cellvibrio gilvus.
作者信息
Hidaka Masafumi, Kitaoka Motomitsu, Hayashi Kiyoshi, Wakagi Takayoshi, Shoun Hirofumi, Fushinobu Shinya
机构信息
Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunko-ku, Tokyo 113-8657, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1877-8. doi: 10.1107/S0907444904017767. Epub 2004 Sep 23.
A recombinant cellobiose phosphorylase from Cellvibrio gilvus has been prepared and crystallized by the sitting-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 1.5 M ammonium sulfate, 0.1 M MES buffer pH 7.0 and 5 mM glucose. A suitable crystal was obtained after 10 d incubation at 298 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 84.77, b = 98.31, c = 104.04 A, beta = 102.73 degrees. X-ray diffraction data to 2.1 A resolution have been collected at KEK-PF BL-5A.
已通过坐滴气相扩散法，使用10 mg/ml纯化酶、1.5 M硫酸铵、0.1 M MES缓冲液（pH 7.0）和5 mM葡萄糖，制备并结晶了来自吉氏纤维弧菌的重组纤维二糖磷酸化酶。在298 K下孵育10天后获得了合适的晶体。该晶体属于空间群P2(1)，晶胞参数为a = 84.77、b = 98.31、c = 104.04 Å，β = 102.73°。已在日本理化所光子工厂BL-5A收集到分辨率为2.1 Å的X射线衍射数据。
Zotero 插件