Porwollik Steffen, Wong Rita Mei-Yee, Helm R Allen, Edwards Kelly K, Calcutt Michael, Eisenstark Abraham, McClelland Michael
Sidney Kimmel Cancer Center, San Diego, California 92121, USA.
J Bacteriol. 2004 Mar;186(6):1678-82. doi: 10.1128/JB.186.6.1678-1682.2004.
Variations in genome size and gene order were observed in archival Salmonella enterica serovar Typhimurium cultures stored for over 40 years. In one strain, microarray analysis revealed a large, stable amplification. PCR analysis of the same strain revealed a genomic duplication that underwent a translocation. Other strains had smaller duplications and deletions. These results demonstrate that storage in stabs over time at room temperature not only allows for further bacterial growth but also may produce an environment that selects for a variety of mutations, including genomic rearrangements.
在保存超过40年的鼠伤寒沙门氏菌档案培养物中观察到基因组大小和基因顺序的变化。在一个菌株中,微阵列分析显示出一个大的、稳定的扩增。对同一菌株的PCR分析揭示了一个发生易位的基因组重复。其他菌株有较小的重复和缺失。这些结果表明,在室温下穿刺保存一段时间不仅允许细菌进一步生长,而且可能产生一个选择各种突变(包括基因组重排)的环境。