Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85718, USA.
J Bacteriol. 2010 Jan;192(2):560-7. doi: 10.1128/JB.01233-09. Epub 2009 Nov 6.
Salmonella enterica serovar Typhimurium is a Gram-negative pathogen that causes gastroenteritis in humans and a typhoid-like disease in mice and is often used as a model for the disease promoted by the human-adapted S. enterica serovar Typhi. Despite its health importance, the only S. Typhimurium strain for which the complete genomic sequence has been determined is the avirulent LT2 strain, which is extensively used in genetic and physiologic studies. Here, we report the complete genomic sequence of the S. Typhimurium strain 14028s, as well as those of its progenitor and two additional derivatives. Comparison of these S. Typhimurium genomes revealed differences in the patterns of sequence evolution and the complete inventory of genetic alterations incurred in virulent and avirulent strains, as well as the sequence changes accumulated during laboratory passage of pathogenic organisms.
鼠伤寒沙门氏菌血清型 Typhimurium 是一种革兰氏阴性病原体，可导致人类肠胃炎和类似于伤寒的疾病，常被用作人类适应的鼠伤寒沙门氏菌血清型 Typhi 引起的疾病模型。尽管它具有重要的健康意义，但唯一完成全基因组测序的鼠伤寒沙门氏菌菌株是无毒 LT2 菌株，该菌株广泛用于遗传和生理研究。在这里，我们报告了鼠伤寒沙门氏菌 14028s 菌株的完整基因组序列，以及其前体和另外两个衍生菌株的基因组序列。对这些鼠伤寒沙门氏菌基因组的比较揭示了毒力和无毒菌株中序列进化模式和完整遗传改变清单的差异，以及在致病生物体的实验室传代过程中积累的序列变化。
