Eriksson A M, Lundgren B, Andersson K, DePierre J W
Department of Biochemistry, Wallenberg Laboratory, Stockholm University, Sweden.
FEBS Lett. 1992 Aug 17;308(2):211-4. doi: 10.1016/0014-5793(92)81276-r.
Dietary treatment of male C57B1/6 mice with clofibrate, nafenopin or WY-14.643 resulted in a modest (at most 2-fold) increase in the total catalase activity in the whole homogenate and mitochondrial fraction prepared from the livers of these animals. On the other hand, the catalase activity recovered in the cytosolic fraction was increased 12- to 18-fold, i.e. 30-35% of the total catalase activity in the hepatic homogenate was present in the high-speed supernatant fraction after treatment with these peroxisome proliferators. A study of the time course of the changes in peroxisomal and cytosolic catalase activities demonstrated that the peroxisomal activity both increased upon initiation of exposure and decreased after termination of treatment several days after the increase and decrease, respectively, in the corresponding cytosolic activity. This finding suggests that the cytosolic catalase may be on its way to incorporation into peroxisomes.
用氯贝丁酯、安妥明或WY-14.643对雄性C57B1/6小鼠进行饮食治疗，导致这些动物肝脏制备的全匀浆和线粒体部分中的总过氧化氢酶活性适度增加（最多2倍）。另一方面，胞质部分中恢复的过氧化氢酶活性增加了12至18倍，即在用这些过氧化物酶体增殖剂处理后，肝匀浆中总过氧化氢酶活性的30%至35%存在于高速上清液部分。对过氧化物酶体和胞质过氧化氢酶活性变化的时间进程研究表明，过氧化物酶体活性在开始暴露时增加，在相应的胞质活性增加和减少几天后，治疗终止后分别下降。这一发现表明，胞质过氧化氢酶可能正在进入过氧化物酶体。
