Sordé Nathalie, Matile Stefan
Department of Organic Chemistry, University of Geneva, Geneva, Switzerland.
Biopolymers. 2004;76(1):55-65. doi: 10.1002/bip.10573.
This report delineates scope and limitation of the selectivity of synthetic multifunctional pores as enzyme sensors using glycolytic enzymes as example (G. Das, P. Talukdar, and S. Matile, Science, 2002, Vol. 298, pp. 1600-1602). Unproblematic detectability of hexokinase and phosphofructokinase demonstrates that the selectivity of synthetic multifunctional pore (SMPs) sensors suffices to sense ATP in mixed analytes containing ADP, whereas detection of the isomerization of glucose 6-phosphate into fructose 6-phosphate by phosphoglucose isomerase is not possible with confidence. The sensitivity of SMP sensors is sufficient for end-point detection of one picomole poly-L-glutamate hydrolyzed by papain in unoptimized assay format; the sensitivity of melittin as representative biological pore of similar charge and aggregation number to detect the same reaction is more than four orders of magnitude inferior.
本报告以糖酵解酶为例，阐述了合成多功能孔作为酶传感器的选择性范围和局限性（G. Das、P. Talukdar和S. Matile，《科学》，2002年，第298卷，第1600 - 1602页）。己糖激酶和磷酸果糖激酶的无问题可检测性表明，合成多功能孔（SMPs）传感器的选择性足以在含有ADP的混合分析物中检测ATP，而磷酸葡萄糖异构酶将6 - 磷酸葡萄糖异构化为6 - 磷酸果糖的检测则无法可靠进行。SMP传感器的灵敏度足以在未优化的检测形式下对木瓜蛋白酶水解的一皮摩尔聚 - L - 谷氨酸进行终点检测；作为具有相似电荷和聚集数的代表性生物孔的蜂毒肽检测相同反应的灵敏度则低四个数量级以上。
