Sordé Nathalie, Das Gopal, Matile Stefan
Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Switzerland.
Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):11964-9. doi: 10.1073/pnas.2132894100. Epub 2003 Oct 6.
This report demonstrates that a single set of identical synthetic multifunctional pores can detect the activity of many different enzymes. Enzymes catalyzing either synthesis or degradation of DNA (exonuclease III or polymerase I), RNA (RNase A), polysaccharides (heparinase I, hyaluronidase, and galactosyltransferase), and proteins (papain, ficin, elastase, subtilisin, and pronase) are selected to exemplify this key characteristic of synthetic multifunctional pore sensors. Because anionic, cationic, and neutral substrates can gain access to the interior of complementarily functionalized pores, such pores can be the basis for very user-friendly screening of a broad range of enzymes.
本报告表明，一组相同的合成多功能孔能够检测多种不同酶的活性。选择催化DNA（核酸外切酶III或聚合酶I）、RNA（核糖核酸酶A）、多糖（肝素酶I、透明质酸酶和半乳糖基转移酶）以及蛋白质（木瓜蛋白酶、无花果蛋白酶、弹性蛋白酶、枯草杆菌蛋白酶和链霉蛋白酶）合成或降解的酶来例证合成多功能孔传感器的这一关键特性。由于阴离子、阳离子和中性底物都能够进入功能互补的孔内部，因此这种孔可作为广泛酶类的非常便捷的筛选基础。
