Nikaido O, Fox M
Paterson Laboratories, Christie Hospital, Holt Radium Institute, Manchester M20 9BX, United Kingdom.
Mutat Res. 1976 Jun;35(2):279-87. doi: 10.1016/0027-5107(76)90192-5.
The frequency of surviving colonies in two V79 cell lines exposed to either 6-thioguanine or 8-azaguanine was dependent on initial plating density. Different degrees of metabolic-co-operation were found to occur in the two cell lines and the loss of both spontaneous and added mutants occurred at a lower cell density when 6TG was used for selection than when 8 AZ was used in both cell lines. Both analogues were degraded on incubation in medium plus serum in the absence of added cells. Variation in serum batch had little effect on the rate of degradation or on the frequency of colonies recovered after treatment of V79 cell lines with 8AZ. The reasons for preferring 8AZ to 6TG as a selective agent are discussed.
暴露于6-硫鸟嘌呤或8-氮杂鸟嘌呤的两种V79细胞系中存活集落的频率取决于初始接种密度。发现两种细胞系中发生了不同程度的代谢合作，并且当使用6TG进行选择时，自发突变体和添加突变体的丢失均发生在比使用8AZ时更低的细胞密度下。在没有添加细胞的情况下，将两种类似物在含有血清的培养基中孵育时均会降解。血清批次的变化对降解速率或用8AZ处理V79细胞系后回收的集落频率影响很小。讨论了优先选择8AZ而非6TG作为选择剂的原因。
