Braga P C, Culici M, Ricci D, Dal Sasso M
Department of Pharmacology, School of Medicine, University of Milan, Italy.
J Chemother. 2003 Dec;15(6):543-50. doi: 10.1179/joc.2003.15.6.543.
The aim of this study was to investigate the ability of moxifloxacin to interfere with the mechanism of bacterial adhesion and disrupt the morphological and structural integrity of bacteria. Three Staphylococcus aureus and three Moraxella catarrhalis strains were grown in the presence of 1/2-1/128 minimum inhibitory concentration (MIC) serial dilutions and incubated with human epithelial cells. A significant decrease in adhesion was observed from 1/2 MIC to 1/64 MIC for S. aureus, and from 1/2 MIC to 1/16 MIC for M. catarrhalis. The use of atomic force microscopy, a new technique capable of revealing surface structures in three-dimensional detail and at very high resolution, showed the rapid onset and time course of the sequence of disruptive morphostructural events following the incubation of both S. aureus and M. catarrhalis with sub-MICs of moxifloxacin. Our findings suggest that less than conventional MIC moxifloxacin concentrations may be effective in reducing bacterial adhesiveness and structural integrity on which the maintenance of bacterial activity depends.
本研究的目的是调查莫西沙星干扰细菌黏附机制并破坏细菌形态和结构完整性的能力。将三株金黄色葡萄球菌和三株卡他莫拉菌在1/2 - 1/128最低抑菌浓度（MIC）系列稀释液存在的情况下培养，并与人上皮细胞一起孵育。对于金黄色葡萄球菌，观察到从1/2 MIC到1/64 MIC黏附显著减少，对于卡他莫拉菌，从1/2 MIC到1/16 MIC黏附显著减少。原子力显微镜是一种能够在三维细节上以非常高的分辨率揭示表面结构的新技术，其使用显示了金黄色葡萄球菌和卡他莫拉菌与低于MIC的莫西沙星孵育后，破坏性形态结构事件序列的快速发生和时间进程。我们的研究结果表明，低于传统MIC的莫西沙星浓度可能有效降低细菌黏附性以及细菌活性维持所依赖的结构完整性。
