Gibbons I R, Cosson M P, Evans J A, Gibbons B H, Houck B, Martinson K H, Sale W S, Tang W J
Proc Natl Acad Sci U S A. 1978 May;75(5):2220-4. doi: 10.1073/pnas.75.5.2220.
The motility of demembranated sea urchin sperm flagella and that of embryo cilia reactivated with 0.1 mM ATP are completely inhibited by 4 micron and 0.5 micron vanadium(V) [V(V), in vanadate], respectively. The Mg2+-activated ATPase activity (ATP phosphohydrolase, EC 3.6.1.3)of the latent form of dynein 1 is inhibited 50% by 0.5-1 micron V(V), while the Ca2+-activated ATPase activity is much less sensitive. The inhibition of flagellar beat frequency and of dynein 1 ATPase activity by V(V) appears not to be competitive with ATP. In agreement with other reports, the inhibition of (Na,K)-ATPase by V(V) shows a slow onset in the presence of ATP and is relatively rapid in its absence. With dynein, however, the inhibition occurs at a rapid rate whether or not ATP is present. Catechol at a concentration of 1 mM reverses the V(V) inhibition of reactivated sperm motility, dynein ATPase, and (Na, K)-ATPase. Myosin and actomyosin ATPases show no inhibition by concentrations of V(V) up to 500 micron. The inhibition by V(V) provides a possible technique for distinguishing between the actions of dynein and myosin in different forms of cell motility.
用0.1 mM ATP重新激活的去膜海胆精子鞭毛的运动性以及胚胎纤毛的运动性，分别被4微米和0.5微米的钒（V）[钒酸盐中的V（V）]完全抑制。动力蛋白1潜在形式的Mg2+激活的ATP酶活性（ATP磷酸水解酶，EC 3.6.1.3）被0.5 - 1微米的V（V）抑制50%，而Ca2+激活的ATP酶活性对其敏感性要低得多。V（V）对鞭毛摆动频率和动力蛋白1 ATP酶活性的抑制似乎与ATP不存在竞争性。与其他报道一致，V（V）对（Na，K）-ATP酶的抑制在有ATP存在时起效缓慢，而在无ATP时相对较快。然而，对于动力蛋白，无论是否存在ATP，抑制都快速发生。1 mM浓度的儿茶酚可逆转V（V）对重新激活的精子运动性、动力蛋白ATP酶和（Na，K）-ATP酶的抑制作用。肌球蛋白和肌动球蛋白ATP酶在高达500微米的V（V）浓度下未显示出抑制作用。V（V）的抑制作用为区分动力蛋白和肌球蛋白在不同形式细胞运动中的作用提供了一种可能的技术。
