整合素不参与人类精子与卵膜融合的过程。

Integrins are not involved in the process of human sperm-oolemmal fusion.

作者信息

Sengoku Kazuo, Takuma Naoyuki, Miyamoto Toshinobu, Horikawa Michiharu, Ishikawa Mutsuo

机构信息

Department of Obstetrics and Gynecology, Asahikawa Medical College, Midorigaoka-higashi 2-1, Asahikawa, 0788510, Japan.

出版信息

Hum Reprod. 2004 Mar;19(3):639-44. doi: 10.1093/humrep/deh095. Epub 2004 Jan 29.

DOI:10.1093/humrep/deh095

PMID:14998963

Abstract

BACKGROUND

We investigated whether integrins are required for the human sperm-oocyte binding and fusion processes.

METHODS

The expression of several integrin subunits at the human oocyte plasma membrane was investigated using immunofluorescence microscopy, and the functional role of integrins expressed at the human oocyte surface in sperm-oocyte interaction was studied using a zona-free human oocyte binding and fusion assay. A total of 144 unfertilized oocytes were stained with anti-integrin antibodies and 147 zona-free unfertilized oocytes were inseminated in the presence of various anti-integrin antibodies that were expressed in oocyte plasma membrane.

RESULTS

The antibodies of six alpha integrin subunits (alpha(2), alpha(3), alpha(5), alpha(6), alpha(V), alpha(M)) and six beta integrin subunits (beta(1), beta(2), beta(3), beta(4), beta(5), beta(6)) were bound to the surface of fixed unfertilized oocytes. In contrast, the presence of alpha(1) and alpha(4) subunits could not be verified. The human sperm-oocyte binding was only partially inhibited by blocking antibodies of alpha(2), alpha(3), alpha(5), alpha(6), alpha(V), alpha(M), beta(1), beta(2) and beta(3) with a maximum of 55% inhibition, but antibodies of beta(4), beta(5) and beta(6) showed no effect on sperm-oolemmal binding. A similar reduction of the number of fused sperm was observed. However, the ratio of fused sperm to total sperm (bound and fused) was not impaired by all integrin antibodies, suggesting that integrins had no role in the sperm-oolemmal fusion process.

CONCLUSIONS

These results suggest that one of the binding mechanisms can be inhibited by integrin antibodies but that this mechanism does not play an essential role in the human sperm-oolemmal binding and fusion processes. The other mechanisms, insensitive to integrins, may involve both binding and fusion processes in human oocytes.

摘要

背景

我们研究了整合素是否参与人类精子与卵母细胞的结合及融合过程。

方法

采用免疫荧光显微镜检测几种整合素亚基在人类卵母细胞质膜上的表达，并使用无透明带人类卵母细胞结合与融合试验研究在人类卵母细胞表面表达的整合素在精子 - 卵母细胞相互作用中的功能作用。总共144个未受精的卵母细胞用抗整合素抗体染色，147个无透明带未受精的卵母细胞在存在于卵母细胞质膜中表达的各种抗整合素抗体的情况下进行授精。

结果

六种α整合素亚基（α(2)、α(3)、α(5)、α(6)、α(V)、α(M)）和六种β整合素亚基（β(1)、β(2)、β(3)、β(4)、β(5)、β(6)）的抗体与固定的未受精卵母细胞表面结合。相比之下，α(1)和α(4)亚基的存在无法得到证实。人类精子与卵母细胞的结合仅被α(2)、α(3)、α(5)、α(6)、α(V)、α(M)、β(1)、β(2)和β(3)的阻断抗体部分抑制，最大抑制率为55%，但β(4)、β(5)和β(6)的抗体对精子与卵母细胞膜的结合没有影响。观察到融合精子数量有类似减少。然而，所有整合素抗体均未损害融合精子与总精子（结合和融合）的比例，这表明整合素在精子与卵母细胞膜融合过程中不起作用。