Ferrage Fabien, Eykyn Thomas R, Bodenhausen Geoffrey
Département de Chimie, associé au CNRS Ecole Normale Supérieure, 24 rue Lhomond 75231 Paris, France.
Chemphyschem. 2004 Jan 23;5(1):76-84. doi: 10.1002/cphc.200300905.
Frequency-switched single-transition cross-polarization (FS-ST-CP) provides a versatile tool for selective coherence transfer in heteronuclear NMR of biomolecules such as proteins and nucleic acids. This type of coherence transfer is spin-state-selective and can therefore benefit from the extension of the life-times of selected coherences due to partial cancellation of interfering relaxation mechanisms. The limits of the selectivity of the transfer are discussed by theory and illustrated by experiment. The methods are particularly efficient to obtain quantitative structural and dynamic information for selected residues in medium-sized nitrogen-15 or carbon-13 labeled macromolecules.
频率切换单跃迁交叉极化（FS-ST-CP）为蛋白质和核酸等生物分子的异核核磁共振中的选择性相干转移提供了一种通用工具。这种类型的相干转移是自旋态选择性的，因此由于干扰弛豫机制的部分抵消，选定相干寿命的延长而受益。通过理论讨论了转移选择性的极限，并通过实验进行了说明。这些方法对于获得中等大小的氮-15或碳-13标记大分子中选定残基的定量结构和动力学信息特别有效。
