Studies on structural and immunogenic polypeptides of hydropericardium syndrome virus by SDS-PAGE and western blotting.

作者信息

Kumar Rajesh, Chandra Rajesh

机构信息

Department of Veterinary Microbiology, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263 145, U.S. Nagar, Uttaranchal, India.

出版信息

Comp Immunol Microbiol Infect Dis. 2004 May;27(3):155-61. doi: 10.1016/j.cimid.2003.08.003.

The polypeptide pattern of a local isolate of a virus causing hydropericardium syndrome was analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A total of 12 polypeptides ranging in molecular weight between 13.8 and 110.0 kDa were observed. Western blot analysis of structural polypeptides revealed seven immunogenic polypeptides ranging in molecular weight between 15.8 and 110.0 kDa.