II型禽腺病毒病毒多肽的鉴定与特性分析

Identification and characterization of viral polypeptides from type-II avian adenoviruses.

作者信息

Zhang C L, Nagaraja K V, Sivanandan V, Newman J A

机构信息

Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St Paul 55108.

出版信息

Am J Vet Res. 1991 Jul;52(7):1137-41.

PMID:1654036

Abstract

The polypeptides of serologically related viruses of hemorrhagic enteritis (HE) in turkeys, marble spleen disease (MSD) in pheasants, and splenomegaly in chickens (SMC) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by protein immunoblotting with polyclonal antibodies to HE virus (HEV). The viral polypeptides II, III, IV, V, VI, and VII were detected on SDS-PAGE with the size range from 18 to 97 kDa in HEV. Viral polypeptides II, III, V, VI, and VII were detected in MSD virus and virus of SMC. Protein immunoblotting of viral proteins with anti-HEV serum revealed antigenic differences between the 3 viruses of avian adenovirus type-II examined. The differences were that the polypeptides II, III, IV, V, VI, and VII were identified in HEV and the polypeptides II, V, VI, and VII were identified in MSD virus and virus of SMC. The bands of penton base (polypeptide III) and fiber (polypeptide IV) were seen in HEV only by protein immunoblotting.

摘要

通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离了火鸡出血性肠炎（HE）、雉鸡大理石脾病（MSD）和鸡脾肿大（SMC）等血清学相关病毒的多肽，并用抗HE病毒（HEV）的多克隆抗体通过蛋白质免疫印迹法进行分析。在SDS-PAGE上检测到HEV中大小范围为18至97 kDa的病毒多肽II、III、IV、V、VI和VII。在MSD病毒和SMC病毒中检测到病毒多肽II、III、V、VI和VII。用抗HEV血清对病毒蛋白进行蛋白质免疫印迹显示，所检测的3种II型禽腺病毒之间存在抗原差异。差异在于，HEV中鉴定出多肽II、III、IV、V、VI和VII，而MSD病毒和SMC病毒中鉴定出多肽II、V、VI和VII。仅通过蛋白质免疫印迹在HEV中看到五邻体基座（多肽III）和纤维（多肽IV）条带。