Yao Nian-Huan, He Wen-Yi, Lam Kit S, Liu Gang
Chinese Academy of Medical Sciences and Peking Union College of Medicine, Institute of Materia Medica, 1 Xian Nong Tan Street, Beijing 100050, P. R. China.
J Comb Chem. 2004 Mar-Apr;6(2):214-9. doi: 10.1021/cc034005e.
Direct O-glycosylation of amino acids bound to TentaGel resin with a number of glycosyl trichloroacetimidate donors results in high yields. The glycosylation reaction can be easily monitored by analyzing the bead-bound amino acids with high-resolution magic angle spinning (HR-MAS) NMR. These studies pave a new way for the construction of "one-bead one-compound" O-glycopeptide libraries with standard amino acid building blocks and appropriate glycosyl trichloroacetimidate donors.
将多种糖基三氯乙酰亚胺酯供体与结合在TentaGel树脂上的氨基酸进行直接O-糖基化反应，可获得高产率。通过使用高分辨率魔角旋转（HR-MAS）核磁共振分析树脂结合的氨基酸，可轻松监测糖基化反应。这些研究为利用标准氨基酸构建模块和合适的糖基三氯乙酰亚胺酯供体构建“一珠一化合物”O-糖肽文库开辟了一条新途径。
