Cho W-S, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu, Seoul 151-742, South Korea.
J Comp Pathol. 2004 Feb-Apr;130(2-3):112-6. doi: 10.1016/j.jcpa.2003.09.003.
Activated nuclear factor-kappaB (NF-kappaB) and inducible nitric oxide synthase (iNOS) were detected immunohistochemically in pleuropneumonic lungs from 20 pigs naturally infected with Actinobacillus pleuropneumoniae. NF-kappaB was detected mainly in nuclei of inflammatory cells, confirming its activation. Intense immunolabelling for NF-kappaB and iNOS was seen within the lung lesions, but labelling was minimal in unaffected portions of the lung of infected pigs and in normal lung from uninfected (control) pigs. Examination of serial sections from the 20 infected lung samples demonstrated a close association between NF-kappaB and iNOS. This suggests that NF-kappaB plays a key role in triggering the activation of iNOS in porcine pleuropneumonia.
采用免疫组织化学方法，在20头自然感染胸膜肺炎放线杆菌的猪的胸膜肺炎肺组织中检测活化核因子κB（NF-κB）和诱导型一氧化氮合酶（iNOS）。NF-κB主要在炎症细胞的细胞核中检测到，证实其被激活。在肺部病变中可见NF-κB和iNOS的强烈免疫标记，但在感染猪未受影响的肺组织部分以及未感染（对照）猪的正常肺组织中标记极少。对20个感染肺样本的连续切片检查显示NF-κB和iNOS之间密切相关。这表明NF-κB在引发猪胸膜肺炎中iNOS的激活方面起关键作用。
