CD44在犬黑素细胞瘤中的差异表达。

Differential expression of CD44 in canine melanocytic tumours.

作者信息

Serra M, Rabanal R M, Miquel L, Domenzain C, Bassols A

机构信息

Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.

出版信息

J Comp Pathol. 2004 Feb-Apr;130(2-3):171-80. doi: 10.1016/j.jcpa.2003.10.006.

DOI:10.1016/j.jcpa.2003.10.006

PMID:15003475

Abstract

CD44, the main cell surface receptor for hyaluronan (HA), is often overexpressed in tumour cells, and its presence has been related to cell proliferation and migration. Many of the functions of CD44 are mediated through its interaction with hyaluronan. This study investigated the expression of CD44 in CML-1 and CML-10c2 canine melanoma cell lines and melanoma biopsies, and the production of hyaluronan and versican by the canine melanoma cell lines. Versican is an extracellular proteoglycan that binds hyaluronan, forming a tridimensional pericellular coat surrounding the cells. Both canine melanoma cell lines expressed CD44 and produced HA, but only CML-1 produced versican. Cells expressing all three components (CD44, HA and versican) formed abundant extracellular matrices as demonstrated by a particle exclusion assay. CD44 was present within benign and malignant melanomas, but its expression was more intense in malignant melanomas (P < 0.01). In high CD44-expressing tumours, CD44 tended to be present in the periphery of malignant melanomas, whereas its expression was homogeneous in benign melanomas.

摘要

CD44是透明质酸（HA）的主要细胞表面受体，在肿瘤细胞中常过度表达，其存在与细胞增殖和迁移有关。CD44的许多功能是通过其与透明质酸的相互作用介导的。本研究调查了CD44在CML - 1和CML - 10c2犬黑色素瘤细胞系以及黑色素瘤活检组织中的表达，以及犬黑色素瘤细胞系中透明质酸和多功能蛋白聚糖的产生。多功能蛋白聚糖是一种细胞外蛋白聚糖，可结合透明质酸，形成围绕细胞的三维细胞周被。两种犬黑色素瘤细胞系均表达CD44并产生HA，但只有CML - 1产生多功能蛋白聚糖。通过颗粒排除试验证明，表达所有三种成分（CD44、HA和多功能蛋白聚糖）的细胞形成了丰富的细胞外基质。CD44存在于良性和恶性黑色素瘤中，但其在恶性黑色素瘤中的表达更强烈（P < 0.01）。在高表达CD44的肿瘤中，CD44倾向于存在于恶性黑色素瘤的周边，而在良性黑色素瘤中其表达是均匀的。