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一种用于通过聚合酶链反应检测临床样本中结核分枝杆菌复合群的DNA探针的特性分析

Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction.

作者信息

Altamirano M, Kelly M T, Wong A, Bessuille E T, Black W A, Smith J A

机构信息

Department of Pathology, University of British Columbia, Vancouver, Canada.

出版信息

J Clin Microbiol. 1992 Aug;30(8):2173-6. doi: 10.1128/jcm.30.8.2173-2176.1992.

DOI:10.1128/jcm.30.8.2173-2176.1992
PMID:1500529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265465/
Abstract

We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.

摘要

我们克隆并测序了来自结核分枝杆菌的一段DNA片段，用于鉴定结核分枝杆菌复合群的成员。用于培养确认的DNA探针灵敏度和特异性均为100%。通过使用从该探针开发的引物，聚合酶链反应经溴化乙锭染色检测到了20株分枝杆菌。该聚合酶链反应系统在检测200份痰标本中的结核分枝杆菌复合群时，灵敏度为98%，特异性为100%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ac/265465/cbccbffddd17/jcm00032-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ac/265465/046a4f0e2730/jcm00032-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ac/265465/bdaccb5e50e1/jcm00032-0278-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ac/265465/cbccbffddd17/jcm00032-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ac/265465/046a4f0e2730/jcm00032-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ac/265465/bdaccb5e50e1/jcm00032-0278-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20ac/265465/cbccbffddd17/jcm00032-0279-a.jpg

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