Altamirano M, Kelly M T, Wong A, Bessuille E T, Black W A, Smith J A
Department of Pathology, University of British Columbia, Vancouver, Canada.
J Clin Microbiol. 1992 Aug;30(8):2173-6. doi: 10.1128/jcm.30.8.2173-2176.1992.
We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.
我们克隆并测序了来自结核分枝杆菌的一段DNA片段,用于鉴定结核分枝杆菌复合群的成员。用于培养确认的DNA探针灵敏度和特异性均为100%。通过使用从该探针开发的引物,聚合酶链反应经溴化乙锭染色检测到了20株分枝杆菌。该聚合酶链反应系统在检测200份痰标本中的结核分枝杆菌复合群时,灵敏度为98%,特异性为100%。