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通过薄层色谱法(TLC)和高效液相色谱法(HPLC)技术对选定的烟酸衍生物进行色谱分离的比较。

A comparison of chromatographic separation of selected nicotinic acid derivatives by TLC and HPLC techniques.

作者信息

Pyka Alina, Sliwiok Józef, Niestrój Anna

机构信息

Silesian Academy of Medicine, Faculty of Pharmacy, Department of Analytical Chemistry, 4 Jagiellońska Str., 41-200 Sosnowiec, Poland.

出版信息

Acta Pol Pharm. 2003 Sep-Oct;60(5):327-33.

Abstract

The separations of nicotinic acid and its derivatives analyzed by adsorption and reversed phase TLC and HPLC were compared. Results were presented as the dependencies of chromatographic retention parameters of studied compounds on the volume composition [%] of mobile phases. It was shown that there exists the possibility of the separation of compounds into specified groups (adsorption TLC and HPLC) and particular substances within individual groups (reversed phase TLC).

摘要

比较了通过吸附薄层色谱法(TLC)、反相TLC和高效液相色谱法(HPLC)分析的烟酸及其衍生物的分离情况。结果以所研究化合物的色谱保留参数对流动相体积组成[%]的依赖性表示。结果表明,存在将化合物分离成特定组(吸附TLC和HPLC)以及在各个组内分离特定物质(反相TLC)的可能性。

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