高效薄层层析法与高效液相色谱法用于分离和定量生咖啡豆提取物中绿原酸的比较。
Comparison of high performance TLC and HPLC for separation and quantification of chlorogenic acid in green coffee bean extracts.
作者信息
Urakova Irina N, Pozharitskaya Olga N, Shikov Alexander N, Kosman Vera M, Makarov Valery G
机构信息
Interregional Center Adaptogen, St Petersburg, Russia.
出版信息
J Sep Sci. 2008 Feb;31(2):237-41. doi: 10.1002/jssc.200700472.
Two chromatographic methods, high-performance TLC (HPTLC) and HPLC, were developed and used for separation and quantitative determination of chlorogenic acid in green coffee bean extracts. For HPTLC silica gel Kieselgel 60 F 254 plates with ethyl acetate/dichlormethane/formic acid/acetic acid/water (100:25:10:10:11, v/v/v/v/v) as mobile phase were used. Densitometric determination of chlorogenic acid by HPTLC was performed at 330 nm. A gradient RP HPLC method was carried out at 330 nm. All necessary validation tests for both methods were developed for their comparison. There were no statistically significant differences between HPLC and HPTLC for quantitative determination of chlorogenic acid according to the test of equality of the means.
开发了两种色谱方法，即高效薄层色谱法（HPTLC）和高效液相色谱法（HPLC），用于生咖啡豆提取物中绿原酸的分离和定量测定。对于HPTLC，使用硅胶Kieselgel 60 F 254板，以乙酸乙酯/二氯甲烷/甲酸/乙酸/水（100:25:10:10:11，v/v/v/v/v）作为流动相。通过HPTLC在330 nm处进行绿原酸的光密度测定。梯度反相HPLC方法在330 nm处进行。为了比较这两种方法，开展了所有必要的验证试验。根据均值相等性检验，HPLC和HPTLC在绿原酸定量测定方面没有统计学上的显著差异。
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