Connections from the lateral vestibular nucleus to the upper cervical spinal cord of the cat: a study with the anterograde tracer PHA-L.

The projections of neurons in the lateral vestibular nucleus (LVN) to the upper cervical spinal cord of the cat were investigated by means of the anterograde tracer Phaseolus vulgaris leucoagglutinin (PHA-L). At the junction of C1 and C2, axons were distributed bilaterally in the ventromedial funiculi, and ipsilaterally in the ventrolateral and lateral funiculi. The majority of boutons were found ipsilateral to the injection sites and most of these boutons were found at the base of the ventral horn and throughout the medial two-thirds of lamina VIII. A more modest termination zone was found along the ventral border of lamina VII and a small number of boutons were scattered in the dorsal horn. Contralateral termination zones were similar to the ipsilateral projections. There were significant changes in the distribution of vestibulospinal axons and density of boutons at the junction of C3 and C4. At this level, most vestibulospinal axons travelled ipsilaterally and were found along the medial border of the ventromedial funiculus and the ventral margin of the ventrolateral funiculus. The overall distribution of boutons near the border of C3 and C4 was similar to the pattern seen at the junction of C1 and C2. However, bouton density fell by a factor of three. Large zones of the grey matter were devoid of boutons in individual experiments. These results demonstrate that the projections of neurons in the LVN to the upper cervical spinal cord are densest in the regions containing motoneurons supplying suboccipital muscles. This result suggests that monosynaptic connections to those motoneurons may be an important part of the neural circuitry responsible for vestibulocollic reflexes. However, the large number of boutons found in regions dorsal to motoneuron nuclei in all upper cervical segments indicates that the primary path from vestibulospinal axons to neck motoneurons may be indirect and involve relays via spinal interneurons.