非洲爪蟾扭曲原肠胚形成基因的二次鉴定。
Identification of a second Xenopus twisted gastrulation gene.
作者信息
Oelgeschläger Michael, Tran Uyen, Grubisic Kristina, De Robertis Edward M
机构信息
Howard Hughes Medical Institute, and Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, USA.
出版信息
Int J Dev Biol. 2004 Feb;48(1):57-61. doi: 10.1387/ijdb.15005575.
Twisted Gastrulation (Tsg) is a secreted molecule which regulates BMP signalling in the extracellular space as part of an evolutionarily conserved network of interacting proteins. In Xenopus, maternal xTsg mRNA can be found throughout the early embryo. After gastrulation, xTsg is expressed as part of the BMP4 synexpression group until late tadpole stages. Here we report the identification of a second Xenopus Tsg gene (xTsg-2). Xenopus Tsg-2 is highly homologousto xTsg. In particular, amino acid residues which have been shown to be required for the binding of xTsg to BMP and to Chordin are conserved. The expression of Xenopus Tsg-2 mRNA was restricted to late stages of embryonic development; it was detected at tadpole stages in lateral plate mesoderm, neural crest, branchial arches and head mesenchyme. In microinjection experiments, the activity of xTsg-2 mRNA was similar to that of xTsg. We conclude that two Tsg genes act in distinct temporal and spatial territories in the course of Xenopus embryonic development.
扭曲原肠胚形成蛋白（Tsg）是一种分泌分子，作为进化上保守的相互作用蛋白网络的一部分，它在细胞外空间调节骨形态发生蛋白（BMP）信号传导。在非洲爪蟾中，母体xTsg mRNA在整个早期胚胎中都能找到。原肠胚形成后，xTsg作为BMP4共表达组的一部分表达，直至蝌蚪晚期。在此，我们报告了第二个非洲爪蟾Tsg基因（xTsg - 2）的鉴定。非洲爪蟾Tsg - 2与xTsg高度同源。特别是，已证明xTsg与BMP和脊索蛋白结合所必需的氨基酸残基是保守的。非洲爪蟾Tsg - 2 mRNA的表达仅限于胚胎发育后期；在蝌蚪期，在侧板中胚层、神经嵴、鳃弓和头部间充质中检测到该基因。在显微注射实验中，xTsg - 2 mRNA的活性与xTsg相似。我们得出结论，在非洲爪蟾胚胎发育过程中，两个Tsg基因在不同的时间和空间区域发挥作用。
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