Vignal Emmanuel, de Santa Barbara Pascal, Guémar Linda, Donnay Jean Marc, Fort Philippe, Faure Sandrine
Centre de Recherches en Biochimie Macromoléculaire, FRE 2593 CNRS, 1919 route de Mende, 34293 Montpellier, France.
Gene Expr Patterns. 2007 Jan;7(3):282-8. doi: 10.1016/j.modgep.2006.09.002. Epub 2006 Sep 9.
Rho GTPases are signaling components that participate to the control of cell morphology, adhesion and motility through the regulation of F-actin cytoskeleton dynamics. In this paper, we report the identification of RhoB in Xenopus laevis (XRhoB) and its expression pattern during early development. Whole-mount in situ hybridization analysis indicated that XrhoB is expressed at high levels in the dorsal marginal zone early in gastrula and in the dorsal midline at later stages. At mid-neurula stages, XrhoB expression extends to the central nervous system, presomitic mesoderm and somites. Later during development, rhoB mRNA is detected in the eyes, the migrating neural crest cells as well as the dorso-lateral part of the somites.
Rho GTPases是信号传导成分，通过调节F-肌动蛋白细胞骨架动力学参与细胞形态、黏附和运动的控制。在本文中，我们报告了非洲爪蟾（XRhoB）中RhoB的鉴定及其在早期发育过程中的表达模式。整体原位杂交分析表明，XrhoB在原肠胚早期的背侧边缘区高水平表达，在后期阶段表达于背中线。在神经胚中期阶段，XrhoB表达扩展至中枢神经系统、体节中胚层和体节。在发育后期，在眼睛、迁移的神经嵴细胞以及体节的背外侧部分检测到rhoB mRNA。
