Schachar N, McAllister D, Stevenson M, Novak K, McGann L
Department of Surgery, University of Calgary, Alberta, Canada.
J Orthop Res. 1992 Sep;10(5):603-9. doi: 10.1002/jor.1100100502.
To determine the fate of transplanted cryopreserved articular cartilage, an animal model employing the proximal humerus in the rabbit has been developed. Previous studies have been hindered by problems of postoperative joint instability, secondary injury due to immobilization, and paucity of cartilage for analysis. This experiment demonstrates the survival and function of transplanted cartilage by quantitative assessment of metabolic and biochemical parameters. Forty-five New Zealand white rabbits underwent transplantation of the right proximal humerus. In 29 animals, the proximal half of the humerus was resected and replaced by a cryopreserved osteoarticular allograft. Autograft procedures were carried out in the remaining animals. Following sacrifice at 3, 6, 9, and 12 months postoperatively, articular cartilage was analyzed for gross appearance, collagen synthesis, proteoglycan synthesis, and water, hydroxyproline, hexosamine, and hexuronic acid contents. The results indicate that the cryopreserved osteoarticular allografts retained their metabolic and biochemical integrity and behaved as viable and biologically functional units 1 year postoperatively.
为了确定冷冻保存的关节软骨移植后的转归，已建立了一种采用兔肱骨近端的动物模型。先前的研究受到术后关节不稳定、固定导致的继发性损伤以及用于分析的软骨数量不足等问题的阻碍。本实验通过对代谢和生化参数的定量评估来证明移植软骨的存活和功能。45只新西兰白兔接受了右侧肱骨近端移植。在29只动物中，肱骨近端的一半被切除，并用冷冻保存的骨关节同种异体移植物替代。其余动物进行自体移植手术。在术后3、6、9和12个月处死后，对关节软骨进行大体外观、胶原蛋白合成、蛋白聚糖合成以及水、羟脯氨酸、己糖胺和己糖醛酸含量的分析。结果表明，冷冻保存的骨关节同种异体移植物在术后1年保持了其代谢和生化完整性，并表现为有活力的生物功能单位。
