Ryan C M, Yarmush M L, Tompkins R G
Surgical Service, Massachusetts General Hospital, Boston, MA.
J Pharm Sci. 1992 Apr;81(4):350-2. doi: 10.1002/jps.2600810412.
Polyethylene glycol 3350 (PEG 3350) is useful as an orally administered probe to measure in vivo intestinal permeability to macromolecules. Previous methods to detect polyethylene glycol (PEG) excreted in the urine have been hampered by inherent inaccuracies associated with liquid-liquid extraction and turbidimetric analysis. For accurate quantitation by previous methods, radioactive labels were required. This paper describes a method to separate and quantitate PEG 3350 and PEG 400 in human urine that is independent of radioactive labels and is accurate in clinical practice. The method uses sized regenerated cellulose membranes and mixed ion-exchange resin for sample preparation and high-performance liquid chromatography with refractive index detection for analysis. The 24-h excretion for normal individuals after an oral dose of 40 g of PEG 3350 and 5 g of PEG 400 was 0.12 +/- 0.04% of the original dose of PEG 3350 and 26.3 +/- 5.1% of the original dose of PEG 400.
聚乙二醇3350（PEG 3350）可用作口服探针，以测量体内肠道对大分子的通透性。以往检测尿液中排出的聚乙二醇（PEG）的方法，因液 - 液萃取和比浊法分析存在固有误差而受到阻碍。要通过以往方法进行准确定量，需要放射性标记。本文描述了一种在人尿中分离和定量PEG 3350和PEG 400的方法，该方法无需放射性标记，且在临床实践中准确可靠。该方法使用尺寸排阻再生纤维素膜和混合离子交换树脂进行样品制备，并采用示差折光检测的高效液相色谱法进行分析。口服40 g PEG 3350和5 g PEG 400后，正常个体24小时的排泄量分别为PEG 3350初始剂量的0.12±0.04%和PEG 400初始剂量的26.3±5.1%。
