Imaoka A, Setoyama H, Takagi A, Matsumoto S, Umesaki Y
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
J Appl Microbiol. 2004;96(4):656-63. doi: 10.1111/j.1365-2672.2004.02189.x.
Animal models are required for evaluation of the functional foods such as pro/prebiotics exerting effects through the metabolism of the intestinal microflora. The object of this study was to establish new human flora-associated mice reflecting the environment of the human intestinal tract.
We inoculated a human faecal suspension into segmented filamentous bacteria (SFB) monoassociated mice as a model system. In both human flora (HF) and SFB-associated mouse (HF-SFB mouse), intestinal characteristics such as the composition of intraepithelial lymphocytes, the expression of major histocompatibility complex (MHC) class II molecules and the number of immunoglobulin A-producing cells in the mucosa was closer to those of conventionally reared mice than was case with human flora-associated mice (HF mice) lacking SFB. Several predominant bacterial groups except lactobacilli in human flora were found in faeces of HF-SFB mice. Lactobacilli established small populations in the gut of HF-SFB mice when administered before inoculation with the human flora. Faecal enzymatic activities and organic acid concentration of HF-SFB mice proportionally reflected those of the donor subject.
We established a new human flora-associated mouse (HF-SFB mouse), in which intestinal characteristics are normally developed and their major microbial composition reflect the human.
HF-SFB mice are a valuable model for studying pro/prebiotic effects on the human intestine.
动物模型对于评估诸如益生元/益生菌等通过肠道微生物群代谢发挥作用的功能性食品是必需的。本研究的目的是建立反映人类肠道环境的新型人源菌群相关小鼠。
我们将人粪便悬液接种到作为模型系统的分节丝状菌（SFB）单关联小鼠中。在人源菌群（HF）和SFB关联小鼠（HF-SFB小鼠）中，上皮内淋巴细胞组成、主要组织相容性复合体（MHC）II类分子表达以及黏膜中产生免疫球蛋白A的细胞数量等肠道特征，比缺乏SFB的人源菌群相关小鼠（HF小鼠）更接近常规饲养小鼠。在HF-SFB小鼠粪便中发现了人源菌群中除乳酸杆菌外的几个主要细菌类群。在接种人源菌群之前给予乳酸杆菌时，其在HF-SFB小鼠肠道中形成了小种群。HF-SFB小鼠的粪便酶活性和有机酸浓度成比例地反映了供体的情况。
我们建立了一种新型人源菌群相关小鼠（HF-SFB小鼠），其肠道特征正常发育，主要微生物组成反映人类情况。
HF-SFB小鼠是研究益生元/益生菌对人类肠道作用的有价值模型。
