新生小鼠脊髓运动神经元的分离与培养。

Isolation and culture of motor neurons from the newborn mouse spinal cord.

作者信息

Anderson Kirstie N, Potter Allyson C, Piccenna Loretta G, Quah Alvin K J, Davies Kay E, Cheema Surindar S

机构信息

Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.

出版信息

Brain Res Brain Res Protoc. 2004 Feb;12(3):132-6. doi: 10.1016/j.brainresprot.2003.10.001.

DOI:10.1016/j.brainresprot.2003.10.001

PMID:15013463

Abstract

A protocol for the isolation and culture of motor neurons from postnatal day 1 mouse spinal cord is described. After 72 h in culture, phase contrast microscopy reveals healthy cells with motor neuronal morphology and extensive neuritic processes. These neurons express the 75-kDa low-affinity neurotrophin receptor (p75NTR) and choline acetyltransferase (ChAT), both proteins are specifically expressed by neonatal and embryonic motor neurons in vivo. This protocol can be adapted for various postnatal motor neuron assays.

摘要

本文描述了一种从出生后第1天小鼠脊髓中分离和培养运动神经元的方法。培养72小时后，相差显微镜观察到具有运动神经元形态和广泛神经突的健康细胞。这些神经元表达75-kDa低亲和力神经营养因子受体（p75NTR）和胆碱乙酰转移酶（ChAT），这两种蛋白在体内均由新生和胚胎运动神经元特异性表达。该方法可适用于各种新生运动神经元检测。