Krieger C, Kim S U
Department of Medicine, Health Sciences Centre Hospital, University of British Columbia, Vancouver, Canada.
Muscle Nerve. 1991 Jan;14(1):14-21. doi: 10.1002/mus.880140104.
Previously, embryonic tissues have been used to produce monolayer cultures containing mammalian spinal cord neurons (SCN) and motoneurons (MN) for studies of the pathophysiology of motoneuron diseases. We demonstrate here that viable SCN and MN were observed in dissociated cultures from neonatal rat and mouse. These SCN and MN produced neurites and expressed acetylcholinesterase, neuron-specific enolase and neurofilament protein. These results indicate that cultured postnatal SCN and MN are capable of survival, neurite extension, and phenotypic expression in culture.
此前，胚胎组织已被用于培养含有哺乳动物脊髓神经元（SCN）和运动神经元（MN）的单层培养物，以研究运动神经元疾病的病理生理学。我们在此证明，在新生大鼠和小鼠的解离培养物中观察到了存活的SCN和MN。这些SCN和MN产生了神经突，并表达了乙酰胆碱酯酶、神经元特异性烯醇化酶和神经丝蛋白。这些结果表明，培养的出生后SCN和MN能够在培养物中存活、延伸神经突并进行表型表达。
