小鼠发育过程中中枢神经系统内肽链延伸因子eEF1A-1/EF-1α和eEF1A-2/S1转换的免疫特征分析
Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1alpha and eEF1A-2/S1 in the central nervous system during mouse development.
作者信息
Pan Jie, Ruest Louis-Bruno, Xu Suying, Wang Eugenia
机构信息
Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital and Department of Medicine, McGill University, Montréal, Canada.
出版信息
Brain Res Dev Brain Res. 2004 Mar 22;149(1):1-8. doi: 10.1016/j.devbrainres.2003.10.011.
Abstract
During early postnatal development, a switch occurs between eEF1A-1/EF-1alpha and eEF1A-2/S1, homologous peptide elongation factors, in brain, heart, and skeletal muscle; eEF1A-2/S1 becomes the major form expressed in maturity. By immunofluorescent labeling, we detected both homologues in the developing brains of wild-type and wasted mutant mice, carrying a deletion in the eEF1A-2/S1 gene; we found that brain expression of eEF1A-2/S1 protein is restricted to mature, terminally differentiated neurons, and coincides with the disappearance of eEF1A-1/EF-1alpha 20 days after birth. Furthermore, no elongation factor 1A is present in wasted mutant mice neurons following the developmental switch, indicating that the genetic regulation silencing eEF1A-1/EF-1alpha is still functional.
摘要
在出生后早期发育过程中，脑、心脏和骨骼肌中同源的肽链延长因子eEF1A - 1/EF - 1α和eEF1A - 2/S1之间会发生转换；eEF1A - 2/S1成为成熟时表达的主要形式。通过免疫荧光标记，我们在野生型和携带eEF1A - 2/S1基因缺失的消瘦突变小鼠的发育大脑中检测到了这两种同源物；我们发现eEF1A - 2/S1蛋白在大脑中的表达仅限于成熟的终末分化神经元，并且与出生后20天eEF1A - 1/EF - 1α的消失相一致。此外，在发育转换后，消瘦突变小鼠的神经元中不存在延长因子1A，这表明使eEF1A - 1/EF - 1α沉默的基因调控仍然有效。
相似文献
1
Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1alpha and eEF1A-2/S1 in the central nervous system during mouse development.
Brain Res Dev Brain Res. 2004 Mar 22;149(1):1-8. doi: 10.1016/j.devbrainres.2003.10.011.
2
Characterization of elongation factor-1A (eEF1A-1) and eEF1A-2/S1 protein expression in normal and wasted mice.
J Biol Chem. 2001 Jun 22;276(25):22915-22. doi: 10.1074/jbc.M101011200. Epub 2001 Apr 9.
3
Peptide elongation factor eEF1A-2/S1 expression in cultured differentiated myotubes and its protective effect against caspase-3-mediated apoptosis.
J Biol Chem. 2002 Feb 15;277(7):5418-25. doi: 10.1074/jbc.M110685200. Epub 2001 Nov 27.
4
Changes in protein levels of elongation factors, eEF1A-1 and eEF1A-2/S1, in long-term denervated rat muscle.
Restor Neurol Neurosci. 2003;21(1-2):47-53.
5
The lethal mutation of the mouse wasted (wst) is a deletion that abolishes expression of a tissue-specific isoform of translation elongation factor 1alpha, encoded by the Eef1a2 gene.
Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4463-8. doi: 10.1073/pnas.95.8.4463.
6
Terminal differentiation-dependent alteration in the expression of translation elongation factor-1 alpha and its sister gene, S1, in neurons.
Exp Cell Res. 1995 Aug;219(2):589-97. doi: 10.1006/excr.1995.1268.
7
Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor 1A.
J Biol Chem. 2017 Sep 29;292(39):16014-16023. doi: 10.1074/jbc.M117.801035. Epub 2017 Aug 11.
8
HIV-1 Uncoating and Reverse Transcription Require eEF1A Binding to Surface-Exposed Acidic Residues of the Reverse Transcriptase Thumb Domain.
mBio. 2018 Mar 27;9(2):e00316-18. doi: 10.1128/mBio.00316-18.
9
Mutational analysis reveals potential phosphorylation sites in eukaryotic elongation factor 1A that are important for its activity.
FEBS Lett. 2021 Sep;595(17):2208-2220. doi: 10.1002/1873-3468.14164. Epub 2021 Jul 31.
10
Structural basis for nucleotide exchange and competition with tRNA in the yeast elongation factor complex eEF1A:eEF1Balpha.
Mol Cell. 2000 Nov;6(5):1261-6. doi: 10.1016/s1097-2765(00)00122-2.
引用本文的文献
1
Local mitochondrial replication in the periphery of neurons requires the eEF1A1 protein and thetranslation of nuclear-encoded proteins.
iScience. 2024 Feb 5;27(4):109136. doi: 10.1016/j.isci.2024.109136. eCollection 2024 Apr 19.
2
Face-valid phenotypes in a mouse model of the most common mutation in EEF1A2-related neurodevelopmental disorder.
Dis Model Mech. 2024 Jun 1;17(6). doi: 10.1242/dmm.050501. Epub 2024 Feb 2.
3
Oncogenic activation of EEF1A2 expression: a journey from a putative to an established oncogene.
Cell Mol Biol Lett. 2024 Jan 3;29(1):6. doi: 10.1186/s11658-023-00519-9.
4
Insulin-Like Growth Factor-1 Supplementation Promotes Brain Maturation in Preterm Pigs.
eNeuro. 2023 Apr 14;10(4). doi: 10.1523/ENEURO.0430-22.2023. Print 2023 Apr.
5
Analysis of the Expression and Subcellular Distribution of and mRNAs during Neurodevelopment.
Cells. 2022 Jun 9;11(12):1877. doi: 10.3390/cells11121877.
6
On the Need to Tell Apart Fraternal Twins eEF1A1 and eEF1A2, and Their Respective Outfits.
Int J Mol Sci. 2021 Jun 28;22(13):6973. doi: 10.3390/ijms22136973.
7
Cell-type and fetal-sex-specific targets of prenatal alcohol exposure in developing mouse cerebral cortex.
iScience. 2021 Apr 20;24(5):102439. doi: 10.1016/j.isci.2021.102439. eCollection 2021 May 21.
8
Damaging de novo missense variants in EEF1A2 lead to a developmental and degenerative epileptic-dyskinetic encephalopathy.
Hum Mutat. 2020 Jul;41(7):1263-1279. doi: 10.1002/humu.24015. Epub 2020 Apr 6.
9
The expression profile and prognostic significance of eukaryotic translation elongation factors in different cancers.
PLoS One. 2018 Jan 17;13(1):e0191377. doi: 10.1371/journal.pone.0191377. eCollection 2018.
10
MLIF Alleviates SH-SY5Y Neuroblastoma Injury Induced by Oxygen-Glucose Deprivation by Targeting Eukaryotic Translation Elongation Factor 1A2.
PLoS One. 2016 Feb 26;11(2):e0149965. doi: 10.1371/journal.pone.0149965. eCollection 2016.
本文引用的文献
1
Peptide elongation factor eEF1A-2/S1 expression in cultured differentiated myotubes and its protective effect against caspase-3-mediated apoptosis.
J Biol Chem. 2002 Feb 15;277(7):5418-25. doi: 10.1074/jbc.M110685200. Epub 2001 Nov 27.
2
Characterization of elongation factor-1A (eEF1A-1) and eEF1A-2/S1 protein expression in normal and wasted mice.
J Biol Chem. 2001 Jun 22;276(25):22915-22. doi: 10.1074/jbc.M101011200. Epub 2001 Apr 9.
3
Rapid up-regulation of peptide elongation factor EF-1alpha protein levels is an immediate early event during oxidative stress-induced apoptosis.
Exp Cell Res. 2000 Aug 25;259(1):140-8. doi: 10.1006/excr.2000.4952.
4
Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells.
J Cell Biol. 1998 Dec 14;143(6):1471-84. doi: 10.1083/jcb.143.6.1471.
5
Eukaryotic translation elongation factor 1 alpha: structure, expression, functions, and possible role in aminoacyl-tRNA channeling.
Prog Nucleic Acid Res Mol Biol. 1998;60:47-78. doi: 10.1016/s0079-6603(08)60889-2.
6
Purification and characterisation of a tissue specific elongation factor 1 alpha (EF-1 alpha 2) from rabbit muscle.
Biochem Biophys Res Commun. 1998 Apr 28;245(3):810-4. doi: 10.1006/bbrc.1998.8510.
7
The lethal mutation of the mouse wasted (wst) is a deletion that abolishes expression of a tissue-specific isoform of translation elongation factor 1alpha, encoded by the Eef1a2 gene.
Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4463-8. doi: 10.1073/pnas.95.8.4463.
8
The elongation factor 1 A-2 isoform from rabbit: cloning of the cDNA and characterization of the protein.
Nucleic Acids Res. 1998 Apr 15;26(8):1884-90. doi: 10.1093/nar/26.8.1884.
9
RNA polymerase of vesicular stomatitis virus specifically associates with translation elongation factor-1 alphabetagamma for its activity.
Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1449-54. doi: 10.1073/pnas.95.4.1449.
10
Apoptosis rate can be accelerated or decelerated by overexpression or reduction of the level of elongation factor-1 alpha.
Exp Cell Res. 1998 Jan 10;238(1):168-76. doi: 10.1006/excr.1997.3819.
Zotero 插件