炭疽菌中的葡糖神经酰胺参与分生孢子向菌丝细胞的分化过程。

Glucosylceramides in Colletotrichum gloeosporioides are involved in the differentiation of conidia into mycelial cells.

作者信息

da Silva André F C, Rodrigues Marcio L, Farias Sandra E, Almeida Igor C, Pinto Márcia R, Barreto-Bergter Eliana

机构信息

Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Cidade Universitária, Rio de Janeiro 21941-590, Brazil.

出版信息

FEBS Lett. 2004 Mar 12;561(1-3):137-43. doi: 10.1016/S0014-5793(04)00156-5.

DOI:10.1016/S0014-5793(04)00156-5

PMID:15013765

Abstract

Glucosylceramides (GlcCer) were extracted from the plant pathogen Colletotrichum gloeosporioides and purified by several chromatographic steps. By using electrospray ionization mass spectrometry and nuclear magnetic resonance, GlcCer from C. gloeosporioides were identified as N-2'-hydroxyoctadecanoyl-1-beta-D-glucopyranosyl-9-methyl-4,8-sphingadienine and N-2'-hydroxyoctadecenoyl-1-beta-D-glucopyranosyl-9-methyl-4,8-sphingadienine. Monoclonal antibodies against these structures were produced and used as tools for the evaluation of the role of GlcCer in the morphological transition of C. gloeosporioides. In the presence of antibodies to GlcCer, the differentiation of conidia into mycelia was blocked. Since GlcCer is present in several plant pathogens, the inhibitory activity of external ligands recognizing these structures may be applicable in other models of fungal infections.

摘要

从植物病原菌胶孢炭疽菌中提取了葡糖神经酰胺（GlcCer），并通过多个色谱步骤进行纯化。利用电喷雾电离质谱法和核磁共振技术，将胶孢炭疽菌中的GlcCer鉴定为N-2'-羟基十八烷酰基-1-β-D-吡喃葡萄糖基-9-甲基-4,8-鞘氨二烯和N-2'-羟基十八碳烯酰基-1-β-D-吡喃葡萄糖基-9-甲基-4,8-鞘氨二烯。制备了针对这些结构的单克隆抗体，并将其用作评估GlcCer在胶孢炭疽菌形态转变中作用的工具。在存在GlcCer抗体的情况下，分生孢子向菌丝体的分化受到阻碍。由于GlcCer存在于多种植物病原菌中，识别这些结构的外部配体的抑制活性可能适用于其他真菌感染模型。