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胶束电动色谱法测定车前属植物中的桃叶珊瑚苷和梓醇

Determination of aucubin and catalpol in Plantago species by micellar electrokinetic chromatography.

作者信息

Jurisić Renata, Debeljak Zeljko, Vladimir-Knezević Sanda, Vuković Jadranka

机构信息

Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovacića 1, HR-10000 Zagreb, Croatia.

出版信息

Z Naturforsch C J Biosci. 2004 Jan-Feb;59(1-2):27-31. doi: 10.1515/znc-2004-1-206.

Abstract

Micellar electrokinetic chromatography (MEKC) was used for the separation and determination of two iridoid glycosides, aucubin and catalpol, in several Plantago species growing in Croatia: P. altissima L., P. argentea Chaix, P. coronopus L., P. holosteum Scop. (subsp. depauperata, subsp. holosteum and subsp. scopulorum), P. lagopus L., P. lanceolata L., and P. maritima L. Hot water extraction (HWE) was applied for the isolation of iridoid substances. Significant differences appeared between the iridoid contents in the examined species. The yield of aucubin and catalpol was up to 0.27% and 1.81% of the dry mass of the leaves, respectively. Besides aucubin and catalpol, two related compounds were determined in the plant samples.

摘要

胶束电动毛细管色谱法(MEKC)用于分离和测定克罗地亚生长的几种车前草属植物中的两种环烯醚萜苷类化合物,即桃叶珊瑚苷和梓醇,这些植物包括:高车前、银叶车前、海滨车前、硬毛车前(包括矮生亚种、硬毛亚种和岩生亚种)、兔耳车前、披针叶车前和滨海车前。采用热水提取法(HWE)分离环烯醚萜类物质。在所检测的物种中,环烯醚萜含量存在显著差异。桃叶珊瑚苷和梓醇的产量分别高达叶片干质量的0.27%和1.81%。除了桃叶珊瑚苷和梓醇外,还在植物样品中测定了两种相关化合物。

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